Skin

De novo assembly and characterization of skin transcriptome using RNAseq in sheep (Ovis aries)

Y. J. Yue, Liu, J. B., Yang, M., Han, J. L., Guo, T. T., Guo, J., Feng, R. L., and Yang, B. H., De novo assembly and characterization of skin transcriptome using RNAseq in sheep (Ovis aries), vol. 14, pp. 1371-1384, 2015.

Wool is produced via synthetic processes of wool follicles, which are embedded in the skin of sheep. The development of new-generation sequencing and RNA sequencing provides new approaches that may elucidate the molecular regulation mechanism of wool follicle development and facilitate enhanced selection for wool traits through gene-assisted selection or targeted gene manipulation. We performed de novo transcriptome sequencing of skin using the Illumina Hiseq 2000 sequencing system in sheep (Ovis aries).

Expressed sequenced tags profiling of resistant and susceptible Gyr x Holstein cattle infested with the tick Rhipicephalus (Boophilus) microplus

C. S. Nascimento, Machado, M. A., Guimarães, S. E. F., Martins, M. F., Peixoto, J. O., Furlong, J., Prata, M. C. A., Verneque, R. S., Teodoro, R. L., and Lopes, P. S., Expressed sequenced tags profiling of resistant and susceptible Gyr x Holstein cattle infested with the tick Rhipicephalus (Boophilus) microplus, vol. 10, pp. 3803-3816, 2011.

Tick resistance in cattle is mainly found in zebu (Bos indicus) animals, although it is also present in some taurine (B. taurus) breeds. In order to characterize functional genes involved in tick resistance/susceptibility in cattle, two cDNA libraries were generated using skin tissues of selected Holstein x Gyr animals. A total of 2700 high-quality reads from both resistant and susceptible cDNA were assembled into 458 sequences (contigs) and 834 singletons, with a mean size of 447.7 nucleotides.

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