In situ hybridization

Expression of the RORα gene in Inner Mongolian cashmere goat hair follicles

Y. H. Zhao, Liu, Z. H., Wang, L., Xiao, H. M., Du, C. G., Zhang, Y. J., Su, R., and Li, J. Q., Expression of the RORα gene in Inner Mongolian cashmere goat hair follicles, vol. 14, pp. 380-388, 2015.

The expression of retinoid-acid-related orphan receptor α (RORα) was evaluated at the mRNA level using real-time polymerase chain reaction (qRT-PCR), and its expression localization was determined by in situ hybridization of adult Inner Mongolian cashmere goats at different times of the year. In situ hybridization demonstrated that RORαwas expressed in secondary hair follicles of the hair shaft, inner root sheath, outer root sheath, medulla, and other parts that are target organs of the RORαreceptor gene.

Cloning and expression analysis of the 37-kDa laminin receptor precursor gene from Hyriopsis cumingii

X. Z. Chang, Li, J. L., Bai, Z. Y., and Li, X. L., Cloning and expression analysis of the 37-kDa laminin receptor precursor gene from Hyriopsis cumingii, vol. 12, pp. 6130-6139, 2013.

Hyriopsis cumingii is an economically important freshwater pearl mussel with high pearl quality that is endemic in China. Investigation of genes relevant to shell formation is important for increased pearl output. The substances that form mollusk shells are secreted by epithelial cells in the mantle, the proliferation of which influences secretion ability. This study focused on the proliferation-related 37-kDa laminin receptor precursor (37LRP) of H. cumingii. The full-length cDNA (1133 bp) encoding this 300-amino acid protein was cloned from the mantle.

Physical location of the carotenoid biosynthesis genes Psy and β-Lcy in Capsicum annuum (Solanaceae) using heterologous probes from Citrus sinensis (Rutaceae)

V. Andrade-Souza, Costa, M. G. C., Chen, C. X., Gmitter, Jr., F. G., and Costa, M. A., Physical location of the carotenoid biosynthesis genes Psy and β-Lcy in Capsicum annuum (Solanaceae) using heterologous probes from Citrus sinensis (Rutaceae), vol. 10, pp. 404-409, 2011.

Carotenoids are responsible for a range of fruit colors in different hot pepper (Capsicum) varieties, from white to deep red. Color traits are genetically determined by three loci, Y, C1, and C2, which are associated with carotenogenic genes. Although such genes have been localized on genetic maps of Capsicum and anchored in Lycopersicon and Solanum, physical mapping in Capsicum has been restricted to only a few clusters of some multiple copy genes.

Expression patterns of the STAG gene in intact and regenerating planarians (Dugesia japonica)

Z. Q. Yuan, Zhao, B. S., and Zhang, J. Y., Expression patterns of the STAG gene in intact and regenerating planarians (Dugesia japonica), vol. 10, pp. 410-418, 2011.

We examined the spatial and temporal expression of the planarian Dugesia japonica STAG-related gene (DjStag), in both intact and regenerating planarians, by whole-mount in situ hybridization and relative quantitative real-time PCR. The first localized transcripts of DjStag were detected in the blastemas three days after amputation, in all regenerates including those from head, tail and trunk pieces. The maximum level of expression of DjStag transcripts occurred at five days after cutting. After regeneration for seven days, DjStag was weakly expressed.

Identification and expression analysis of the Broad-Complex core protein isoform 6 (BR-C Z6) gene in the giant tiger shrimp Penaeus monodon (Penaeidae: Decapoda)

A. Buaklin, Klinbunga, S., and Mensveta, P., Identification and expression analysis of the Broad-Complex core protein isoform 6 (BR-C Z6) gene in the giant tiger shrimp Penaeus monodon (Penaeidae: Decapoda), vol. 10, pp. 2290-2306, 2011.

Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors that function during metamorphosis in insects. We identified two full-length cDNAs of BR-C Z6 in the giant tiger shrimp (Penaeus monodon). They were 2422 and 2060 bp in length, containing open reading frames of 1440 and 1443 bp, corresponding to polypeptides of 479 and 480 amino acids, respectively. Tissue distribution analysis indicated that PmBR-C Z6 was abundantly expressed in hemocytes and ovaries in juveniles.

Molecular cytogenetic characterization of a new leaf rolling triticale

E. N. Yang, Yang, Z. J., Zhang, J. F., Zou, Y. C., and Ren, Z. L., Molecular cytogenetic characterization of a new leaf rolling triticale, vol. 10, pp. 2953-2961, 2011.

Leaf rolling occurs in some cereal genotypes in response to drought. We identified and made a phenotypic, cytological and physiological analysis of a leaf-rolling genotype (CMH83) of hexaploid triticale (X Triticosecale Wittmack) that exhibited reduced plant height, rolled and narrow leaves. Gliadin electrophoresis of seed protein showed that CMH83 was genetically stable. Sequential Giemsa-C-banding and genomic in situ hybridization showed that CMH83 contains 12 rye chromosomes; two pairs of these chromosomes have reduced telomeric heterochromatin bands.

Salt stress causes a shift in the localization pattern of germin gene expression

M. Caliskan, Salt stress causes a shift in the localization pattern of germin gene expression, vol. 8, pp. 1250-1256, 2009.

The response of plants to biotic and abiotic stress factors involves biochemical, physiological, morphological, and developmental changes. Salt stress has been the subject of extensive studies due to the low salt tolerance of many crop plants. Germin and germin-like gene products are known to be involved in various aspects of plant development, such as defense, embryonic development and response to biotic as well as abiotic stress, including salt. The responses of germin and germin-like genes to salt stress vary in different plants.

Localization of HSP single-copy genes by inexpensive, permanent non-fluorescent in situ hybridization on meiotic chromosomes of the grasshopper Schistocerca pallens (Acrididae)

T. T. Rieger, Oliveira-Silva, S. V., Pachêco, I. A., Chagas, B. S., and Santos, J. F., Localization of HSP single-copy genes by inexpensive, permanent non-fluorescent in situ hybridization on meiotic chromosomes of the grasshopper Schistocerca pallens (Acrididae), vol. 6, pp. 643-649, 2007.

There have been many studies on Schistocerca gregaria and Locusta migratoria, which are important grasshopper pests in many parts of the world. However, the main pest grasshopper species in Brazil, S. pallens, Rhammatocerus schistocercoides and Stiphra robusta, are very poorly characterized genetically. We adapted a permanent in situ hybridization method to extend the genetic characterization of S.

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