siRNA

Induction function of siRNA-mediated survivin gene silencing on nasopharyngeal carcinoma cell apoptosis

S. M. Fu, Tu, Z. H., Deng, L. Q., Cai, J. H., Liang, Z., Lin, Z. Q., and Wang, Y. T., Induction function of siRNA-mediated survivin gene silencing on nasopharyngeal carcinoma cell apoptosis, vol. 14, pp. 2537-2545, 2015.

We examined the function of survivin gene expression in patients with nasopharyngeal carcinoma (NPC), as well as small interfering RNA (siRNA) on controlling CNE-2 NPC proliferation and apoptosis. Immunohistological methods, in situ hybridization, and reverse transcription-polymerase chain reaction technique were used to detect survivin protein and mRNA expression. We designed an siRNA sequence to inhibit survivin gene expression. The MTT method was used to examine the function of siRNA on controlling cell growth and proliferation.

Inactivation of Rab23 inhibits the invasion and motility of pancreatic duct adenocarcinoma

Z. Z. Cai, Xu, L. B., Cai, J. L., Wang, J. S., Zhou, B., and Hu, H., Inactivation of Rab23 inhibits the invasion and motility of pancreatic duct adenocarcinoma, vol. 14, pp. 2707-2715, 2015.

Pancreatic ductal adenocarcinoma (PDAC) is charac­terized by a poor prognosis and high mortality rate. In this study, we investigated the expression of Rab23 in non-tumor pancreatic tissues and PDACs via immunohistochemistry. Rab23 was found in 39 of 58 (67.2%) and in 11 of 30 (36.7%) of the PDAC and non-tumor pan­creatic tissue samples (P = 0.0073), respectively. There were signifi­cant correlations between Rab23 expression and unfavorable variables, including cancer differentiation level (P = 0.0089), lymph nodal (P = 0.0099), and distant metastases (P = 0.0173).

Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells

H. Zhang, Chen, F., Li, G. L., Ding, Y. Y., Tao, Z. R., Li, J. J., Zhong, S. L., and Lu, L. Z., Molecular cloning, expression, and regulation of estrogen receptors in pigeon oviduct epithelial cells, vol. 13, pp. 1926-1937, 2014.

Estrogen regulates reproductive behavior and drives the proliferation and differentiation of several cell types. These physiological functions of estrogen are mediated by estrogen receptors (ERs), and each ER isoform plays a distinct role. To clarify the molecular mechanism of estrogen action and to evaluate the effect of ERs on the secretion of ovalbumin (OVA) in pigeon oviduct epithelial cells (POECs), we determined the complete coding sequences encoding ER alpha (ERα) and ER beta (ERβ) in pigeons.

Study on the treatment of the p15 gene combined with Bcr-abl-specific siRNA and STI571 for chronic myeloid leukemia

W. Wang, Du, Y., Li, N. - N., Lv, F. - F., and Lin, G. - Q., Study on the treatment of the p15 gene combined with Bcr-abl-specific siRNA and STI571 for chronic myeloid leukemia, vol. 13, pp. 4089-4101, 2014.

The aim of this study was to investigate the effect of the p15 gene combined with Bcr-abl-specific siRNA and STI571 on the proliferation, cell cycle and apoptosis of K562 chronic myeloid leukemia cells. Using the gene sequence results, we amplified the p15 gene from normal peripheral blood by RT-PCR, and constructed a p15-pcDNA3.1 vector. The K562 cell line with G418 resistance was screened, synthesized and transfected for bcr-abl gene fusion point for 21-nt siRNA.

Effect of BMPRIB gene silencing by siRNA on apoptosis and steroidogenesis of porcine granulosa cells

Y. Y. Zhao, Li, X. X., Wang, W., Chen, X., Yu, P., Wang, J. J., and Xu, Y. X., Effect of BMPRIB gene silencing by siRNA on apoptosis and steroidogenesis of porcine granulosa cells, vol. 13, pp. 9964-9975, 2014.

Bone morphogenetic proteins (BMPs) are the key factors in maintaining cell growth and differentiation in ovaries. BMPs initiate signaling by assembling BMP receptors and activating Smads, which in turn alter the expression of target genes. However, little is known about the effect of the deletion of the Bone morphogenetic protein receptor type IB (BMPRIB) on porcine granulosa cell (GCs).

 Effect of siRNA targeting EZH2 on cell viability and apoptosis of bladder cancer T24 cells

H. F. Wang, Yang, H., Hu, L. B., Lei, Y. H., Qin, Y., Li, J., Bi, C. W., Wang, J. S., and Huo, Q.,  Effect of siRNA targeting EZH2 on cell viability and apoptosis of bladder cancer T24 cells, vol. 13, pp. 9939-9950, 2014.

We investigated the effect of siRNA targeting enhancer of EZH2 on cell proliferation, invasion, migration, and apoptosis of human bladder cancer T24 cells. An siRNA-expressing plasmid targeting the EZH2 gene was transfected into T24 cells. Quantitative polymerase chain reaction and Western blot analysis were used to detect EZH2 expression at the mRNA and protein levels, respectively. Proliferation, invasion, and migration of T24 cells were examined in vivo using MTT, wound healing, and transwell chamber migration assays, respectively.

Knockdown expression of Apc11 leads to cell-cycle distribution reduction in G2/M phase

Y. - J. Shi and Huo, K. - K., Knockdown expression of Apc11 leads to cell-cycle distribution reduction in G2/M phase, vol. 11, pp. 2814-2822, 2012.

Anaphase-promoting complex/cyclosome (APC/C) is a key E3 ubiquitin ligase in cell division, which catalyses ubiquitination of cell-cycle regulators. Studying this complex could reveal important information regarding its application in cancer research and therapy. In this study, 4 synthesized small interfering RNAs (siRNAs) were transfected into HEK293T cells to suppress messenger RNA (mRNA) of Apc11; 2 of these reduced the amount of Apc11 mRNA by over 50%.

Bottom-up assembly of RNA nanoparticles containing phi29 motor pRNA to silence the asthma STAT5b gene

C. Qiu, Peng, W. K., Shi, F., and Zhang, T., Bottom-up assembly of RNA nanoparticles containing phi29 motor pRNA to silence the asthma STAT5b gene, vol. 11, pp. 3236-3245, 2012.

Activation of the transcription factor signal transducer and activator of transcription 5b (STAT5b) is a key event in the development of asthma. The potent ability of small interfering RNA (siRNA) to inhibit the expression of STAT5b mRNA has provided a new class of therapeutics for asthma. However, efficient delivery of siRNAs remains a key obstacle to their successful application. A targeted intracellular delivery approach for siRNA to specific cell types would be highly desirable.

Inhibition of vascular endothelial growth factor A expression in mouse granulosa cells by lentivector-mediated RNAi

N. Ally, Zou, X. L., Jiang, B. C., Qin, L., Zhai, L., Xiao, P., and Liu, H. L., Inhibition of vascular endothelial growth factor A expression in mouse granulosa cells by lentivector-mediated RNAi, vol. 11, pp. 4019-4033, 2012.

Vascular endothelial growth factor (VEGF) has been found responsible for the induction of proliferation and differentiation in granulosa cells. We constructed four short hairpin RNA (shRNA) expression plasmids targeting the mouse VEGFA gene, and examined their effect on VEGF expression in mouse granulosa cells (MGC) in vitro. Four different shRNA oligonucleotides targeting the coding sequence of mouse VEGFA mRNA and one negative control (shNC) were designed and cloned into a pGPU6/GFP/Neo siRNA expression vector, and transiently transfected into MGC.

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