Sinonovacula constricta

Development of polymorphic SSR markers in the razor clam (Sinonovacula constricta) and cross-species amplification

Y. H. Dong, Yao, H. H., Sun, C. S., Lv, D. M., Li, M. Q., Lin, Z. H., Dong, Y. H., Yao, H. H., Sun, C. S., Lv, D. M., Li, M. Q., Lin, Z. H., Dong, Y. H., Yao, H. H., Sun, C. S., Lv, D. M., Li, M. Q., and Lin, Z. H., “Development of polymorphic SSR markers in the razor clam (Sinonovacula constricta) and cross-species amplification”, vol. 15, p. -, 2016.

Next-generation sequencing provides large-scale sequencing data with relative ease and at a reasonable cost, making it possible to identify a large amount of SSR markers in a timely and cost-effective manner. On the basis of the transcriptome database of Sinonovacula constricta obtained by Illumina/Solexa pyrosequencing, 60 polymorphic SSR markers were developed and characterized in 30 individuals. The number of alleles per polymorphic locus ranged from 2 to 7 with an average of 3.75 alleles.

Polymorphic microsatellite loci for the razor clam, Sinonovacula constricta

H. - T. Ma, Jiang, H. - B., Liu, X. - Q., Wu, X. - P., and Wei, X. - M., “Polymorphic microsatellite loci for the razor clam, Sinonovacula constricta”, vol. 14, pp. 145-148, 2015.

The razor clam, Sinonovacula constricta, is an important commercial bivalve and a popular mollusca food in China. Twelve polymorphic microsatellite markers were isolated from the razor clam using a partial genomic library enriched for tandem repeat sequences of (CA)16, (GA)16. Polymorphisms of these loci were evaluated in a wild population of 30 individuals. The allele number of these polymorphic markers ranged from 5-15 per locus with an average of 9.333. Observed and expected heterozygosity values ranged from 0.192-1.000 and 0.219-0.906.

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