Single cell

Sexing single bovine blastomeres using TSPY gene amplification

M. C. A. Carneiro, Takeuchi, P. L., Araújo, A., Lôbo, R. B., Elias, F. P., Vila, R. A., Miranda-Furtado, C. L., and Ramos, E. S., Sexing single bovine blastomeres using TSPY gene amplification, vol. 10, pp. 3937-3941, 2011.

The testis-specific protein Y-encoded gene (TSPY) is a Y-specific gene present in variable copy number in many mammalian species, including cattle. We tested the applicability of the TSPY gene as a Y-specific marker to predict preimplantation embryo sex in Nelore (Bos indicus) cattle. Two blastomeres were removed from each embryo. A total of 36 single blastomeres and the remaining cells of their 18 matched in vitro conceived embryos were screened for TSPY amplification by nested-PCR.

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