Simulated annealing

Optimizing reproducibility evaluation for random amplified polymorphic DNA markers

J. R. Ramos, Telles, M. P. C., Diniz-Filho, J. A. F., Soares, T. N., Melo, D. B., and Oliveira, G., Optimizing reproducibility evaluation for random amplified polymorphic DNA markers, vol. 7, pp. 1384-1391, 2008.

The random amplified polymorphic DNA (RAPD) technique is often criticized because it usually shows low levels of repeatability; thus it can generate spurious bands. These problems can be partially overcome by rigid laboratory protocols and by performing repeatability tests. However, because it is expensive and time-consuming to obtain genetic data twice for all individuals, a few randomly chosen individuals are usually selected for a priori repeatability analysis, introducing a potential bias in genetic parameter estimates.

Subscribe to Simulated annealing