Simple sequence repeat

Transcriptome analysis and de novo annotation of the critically endangered Amur sturgeon (Acipenser schrenckii)

X. J. Zhang, Jiang, H. Y., Li, L. M., Yuan, L. H., Chen, J. P., Zhang, X. J., Jiang, H. Y., Li, L. M., Yuan, L. H., and Chen, J. P., “Transcriptome analysis and de novo annotation of the critically endangered Amur sturgeon (Acipenser schrenckii)”, vol. 15, p. -, 2016.

The aim of this study was to provide comprehensive insights into the genetic background of sturgeon by transcriptome study. We performed a de novo assembly of the Amur sturgeon Acipenser schrenckii transcriptome using Illumina Hiseq 2000 sequencing. A total of 148,817 non-redundant unigenes with base length of approximately 121,698,536 bp and ranges from 201 to 26,789 bp were obtained. All the unigenes were classified into 3368 distinct categories and 145,449 singletons by homologous transcript cluster analysis.

Isolation and characterization of microsatellite markers for Eucommia ulmoides (Eucommiaceae), an endangered tree, using next-generation sequencing

W. R. Zhang, Li, Y., Zhao, J., Wu, C. H., Ye, S., Yuan, W. J., Zhang, W. R., Li, Y., Zhao, J., Wu, C. H., Ye, S., Yuan, W. J., Zhang, W. R., Li, Y., Zhao, J., Wu, C. H., Ye, S., and Yuan, W. J., “Isolation and characterization of microsatellite markers for Eucommia ulmoides (Eucommiaceae), an endangered tree, using next-generation sequencing”, vol. 15, p. -, 2016.

Eucommia ulmoides Oliver, a single extant species of Eucommiaceae, is an endemic dioecious tree in China. The natural resources of E. ulmoides have rapidly declined in recent years because of the over-collection of its cortex. To design a suitable protection strategy, it is necessary to develop a set of molecular markers to investigate genetic diversity and population structure of E. ulmoides. Pyrosequencing of an enriched microsatellite library by Roche 454 FLX+ platform was used to isolate simple sequence repeats (SSRs) for E. ulmoides.

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