Simple sequence repeat

Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep

M. Yang, Yue, Y. J., Guo, T. T., Han, J. L., Liu, J. B., Guo, J., Sun, X. P., Feng, R. L., Wu, Y. Y., Wang, C. F., Wang, L. P., and Yang, B. H., Limitation of high-resolution melting curve analysis for genotyping simple sequence repeats in sheep, vol. 13, pp. 2645-2653, 2014.

Variation in microsatellite or simple sequence repeat (SSR) loci has, until recently, relied heavily on the use of gel-based methods that can be both time consuming and difficult to genotype. Non gel-based systems are therefore important to increase simplicity and improve turn-around time without compromising assay sensitivity and accuracy. In this report, we assessed the latest of the non-gel-based methods, high-resolution melting (HRM) curve analysis.

Analysis of sequence diversity through internal transcribed spacers and simple sequence repeats to identify Dendrobium species

Y. T. Liu, Chen, R. K., Lin, S. J., Chen, Y. C., Chin, S. W., Chen, F. C., and Lee, C. Y., Analysis of sequence diversity through internal transcribed spacers and simple sequence repeats to identify Dendrobium species, vol. 13, pp. 2709-2717, 2014.

The Orchidaceae is one of the largest and most diverse families of flowering plants. The Dendrobium genus has high economic potential as ornamental plants and for medicinal purposes. In addition, the species of this genus are able to produce large crops. However, many Dendrobium varieties are very similar in outward appearance, making it difficult to distinguish one species from another. This study demonstrated that the 12 Dendrobium species used in this study may be divided into 2 groups by internal transcribed spacer (ITS) sequence analysis.

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