shRNA

Inhibition of vascular endothelial growth factor A expression in mouse granulosa cells by lentivector-mediated RNAi

N. Ally, Zou, X. L., Jiang, B. C., Qin, L., Zhai, L., Xiao, P., and Liu, H. L., Inhibition of vascular endothelial growth factor A expression in mouse granulosa cells by lentivector-mediated RNAi, vol. 11, pp. 4019-4033, 2012.

Vascular endothelial growth factor (VEGF) has been found responsible for the induction of proliferation and differentiation in granulosa cells. We constructed four short hairpin RNA (shRNA) expression plasmids targeting the mouse VEGFA gene, and examined their effect on VEGF expression in mouse granulosa cells (MGC) in vitro. Four different shRNA oligonucleotides targeting the coding sequence of mouse VEGFA mRNA and one negative control (shNC) were designed and cloned into a pGPU6/GFP/Neo siRNA expression vector, and transiently transfected into MGC.

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