Sex determination

Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim.

S. H. Wang, Li, Y., Li, Z. Q., Chang, L., and Li, L., “Identification of an SCAR marker related to female phenotype in Idesia polycarpa Maxim.”, vol. 14, pp. 2015-2022, 2015.

Idesia polycarpa Maxim. is a dioecious species. Because of the lack of morphological and cytological methods available for identifying its sex during the long juvenile stage, the application of molecular markers in sex identification may facilitate sex determination in the seedling stage. The objective of this study was to use sequence-related amplified polymorphism to identify sex-linked markers in I. polycarpa and convert these markers into sequence-characterized amplified region markers, which are much easier to identify.

SCAR marker for sex identification of Pistacia chinensis Bunge (Anacardiaceae)

Q. Sun, Yang, X., and Li, R., “SCAR marker for sex identification of Pistacia chinensis Bunge (Anacardiaceae)”, vol. 13, pp. 1395-1401, 2014.

Pistacia chinensis Bunge is a dioecious plant that originated in China, and its sex cannot be identified at the early stage of cultivation by only its appearance. Recent studies show that the seed of P. chinensis is an ideal feedstock for biofuel production. To guide the cultivation of this energy plant scientifically, a new method is urgently needed to identify the sex of P. chinensis seedlings.

Analysis of interaction between Bmhrp28 and BmPSI in sex-specific splicing of Bombyx mori Bmdsx gene

X. F. Zha, Zhao, M., Zhou, C. Y., Guo, H. Z., Zhao, P., Xiang, Z. H., and Xia, Q. Y., “Analysis of interaction between Bmhrp28 and BmPSI in sex-specific splicing of Bombyx mori Bmdsx gene”, vol. 13, pp. 5452-5462, 2014.

Bombyx mori BmHRP28 and BmPSI, which belong to the family of RNA-binding proteins, have been identified binding to the female-specific exon 4 of the sex-determining gene Bmdsx pre-mRNA. However, the relationships between BmHRP28 and BmPSI still remain unclear. In this study, we carried out yeast two-hybrid (Y2H) and co-immunoprecipitation (Co-IP) analyses to address them. Y2H analysis showed that there was little or no direct binding between the BmHRP28 and BmPSI proteins.

Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense

Y. P. Zhang, Qiao, H., Zhang, W. Y., Sun, S. M., Jiang, S. F., Gong, Y. S., Xiong, Y. W., Jin, S. B., and Fu, H. T., “Molecular cloning and expression analysis of two sex-lethal homolog genes during development in the oriental river prawn, Macrobrachium nipponense”, vol. 12, pp. 4698-4711, 2013.

In this study, two Sxl gene homologs, designated as Mnsxl1 and Mnsxl2, were cloned and characterized from the freshwater prawn Macrobrachium nipponense by rapid amplification of cDNA ends. The deduced amino acid sequences of Mnsxl1 and Mnsxl2 showed high sequence homology to the insect Sxl and contained conserved domains in two RNA-binding motifs.

Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers

I. G. Almeida, Ianella, P., Faria, M. T., Paiva, S. R., and Caetano, A. R., “Bulked segregant analysis of the pirarucu (Arapaima gigas) genome for identification of sex-specific molecular markers”, vol. 12, pp. 6299-6308, 2013.

Arapaima gigas (Osteoglossidae) is one of the largest fish species in the Amazon Basin, attaining lengths of over 2.5 m and weights of over 100 kg. Its flesh is prized, and it has great potential for production in aquaculture systems. However, live pirarucu cannot be reliably sexed visually, even after sexual development, since this species does not have clear external sexual dimorphism. Simple and inexpensive methods for sexing immature pirarucu based on DNA markers would facilitate production of this species in commercial operations. We analyzed A.

A comparative analysis of highly conserved sex-determining genes between Apis mellifera and Drosophila melanogaster

A. S. Cristino, Nascimento, A. Mendes do, Costa, Lda Fontour, and Simões, Z. Luz Paulin, “A comparative analysis of highly conserved sex-determining genes between Apis mellifera and Drosophila melanogaster”, vol. 5, pp. 154-168, 2006.

A comparison of the most conserved sex-determining genes between the fruit fly, Drosophila melanogaster, and the honey bee, Apis mellifera, was performed with bioinformatics tools developed for computational molecular biology.

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