Sequencing

Genes expressed in cotton (Gossypium hirsutum) buds isolated with a subtractive library

M. P. N. Pinheiro, Batista, V. G. L., Martins, N. F., Santos, R. C., Filho, P. A. Melo, Silva, C. R. C., and Lima, L. M., Genes expressed in cotton (Gossypium hirsutum) buds isolated with a subtractive library, vol. 12, pp. 37-43, 2013.

A subtractive cDNA library from cotton buds was constructed to prospect for differentially expressed genes related to early bud development. A library was constructed and 768 cDNA sequences were obtained, comprising 168 clusters, with 126 contigs and 42 singlets. Both the Gossypium as well as Arabidopsis databases were utilized for the in silico analysis, since some genes identified in cotton have not yet been studied for functionality, although they have homology with genes from other species.

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