Sequence-characterized amplified region (SCAR)

Development of novel SCAR markers for genetic characterization of Lonicera japonica from high GC-RAMP-PCR and DNA cloning

J. L. Cheng, Li, J., Qiu, Y. M., Wei, C. L., Yang, L. Q., Fu, J. J., Cheng, J. L., Li, J., Qiu, Y. M., Wei, C. L., Yang, L. Q., Fu, J. J., Cheng, J. L., Li, J., Qiu, Y. M., Wei, C. L., Yang, L. Q., and Fu, J. J., Development of novel SCAR markers for genetic characterization of Lonicera japonica from high GC-RAMP-PCR and DNA cloning, vol. 15, p. -, 2016.

Sequence-characterized amplified region (SCAR) markers were further developed from high-GC primer RAMP-PCR-amplified fragments from Lonicera japonica DNA by molecular cloning. The four DNA fragments from three high-GC primers (FY-27, FY-28, and FY-29) were successfully cloned into a pGM-T vector. The positive clones were sequenced; their names, sizes, and GenBank numbers were JYHGC1-1, 345 bp, KJ620024; YJHGC2-1, 388 bp, KJ620025; JYHGC7-2, 1036 bp, KJ620026; and JYHGC6-2, 715 bp, KJ620027, respectively.

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