Due to the morphological similarities of aerial parts, it is difficult to distinguish Gynostemma pentaphyllum from Cayratia japonica, which is usually an adulterant of the former. To develop a reliable method for the identification and authentication of G. pentaphyllum, a combination of random amplification polymorphic DNA (RAPD) technique with sequence-characterized amplified region (SCAR) markers was studied. Twenty-five samples of G. pentaphyllum and two samples of C.
Sequence-characterized amplified region
The sequence-characterized amplified region (SCAR) is a valuable molecular technique for the genetic identification of any species. This method is mainly derived from the molecular cloning of the amplified DNA fragments achieved from the random amplified polymorphic DNA (RAPD). In this study, we collected DNA from 10 species of Ganoderma mushroom and amplified the DNA using an improved RAPD technique. The amplified fragments were then cloned into a T-vector, and positive clones were screened, indentified, and sequenced for the development of SCAR markers.
Pistacia chinensis Bunge is a dioecious plant that originated in China, and its sex cannot be identified at the early stage of cultivation by only its appearance. Recent studies show that the seed of P. chinensis is an ideal feedstock for biofuel production. To guide the cultivation of this energy plant scientifically, a new method is urgently needed to identify the sex of P. chinensis seedlings.