Sequence analysis

Cloning and sequence analysis of sucrose phosphate synthase gene from varieties of Pennisetum species

H. C. Li, Lu, H. B., Yang, F. Y., Liu, S. J., Bai, C. J., and Zhang, Y. W., “Cloning and sequence analysis of sucrose phosphate synthase gene from varieties of Pennisetum species”, vol. 14, pp. 2799-2808, 2015.

Sucrose phosphate synthase (SPS) is an enzyme used by higher plants for sucrose synthesis. In this study, three primer sets were designed on the basis of known SPS sequences from maize (GenBank: NM_001112224.1) and sugarcane (GenBank: JN584485.1), and five novel SPS genes were identified by RT-PCR from the genomes of Pennisetum spp (the hybrid P. americanum x P. purpureum, P. purpureum Schum., P. purpureum Schum. cv. Red, P. purpureum Schum. cv. Taiwan, and P. purpureum Schum. cv. Mott).

SRAP analysis of DNA base sequence changes in lotus mutants induced by Fe+ implantation

C. L. Deng, Qin, R. Y., Gao, J., Jia, Y. Y., Ren, Y. X., Gao, W. J., and Lu, L. D., “SRAP analysis of DNA base sequence changes in lotus mutants induced by Fe+ implantation”, vol. 12, pp. 335-343, 2013.

Ion implantation, a new biophysically mutagenic technique, has shown great potential for crop breeding. To reveal the mutation effect of low-energy ion implantation on Baiyangdian red lotus, sequence-related amplified polymorphism markers were used to amplify and detect the DNA sequence differences in mutants induced by Fe+ ion implantation. A total of 121 primer combinations were tested in 6 mutants and a control.

Molecular cloning and functional analysis of MRLC2 in Tianfu, Boer, and Chengdu Ma goats

H. G. Xu, Xu, G. Y., Wan, L., and Ma, J., “Molecular cloning and functional analysis of MRLC2 in Tianfu, Boer, and Chengdu Ma goats”, vol. 12, pp. 3510-3520, 2013.

To determine the molecular basis of heterosis in goats, fluorescence quantitative polymerase chain reaction (PCR) was performed to investigate myosin-regulatory light chain 2 (MRLC2) gene expression in the longissimus dorsi muscle tissues of the Tianfu goat and its parents, the Boer and Chengdu Ma goats. The goat MRLC2 gene was differentially expressed in the crossbreed, and the purebred mRNA were isolated and identified using fluorescence quantitative reverse transcription-PCR (RT-PCR).

cDNA, genomic sequence cloning and overexpression of the ribosomal protein S13 gene in the giant panda (Ailuropoda melanoleuca)

Y. Song, Hou, Y. - L., Hou, W. - R., Wu, G. - F., and Zhang, T., “cDNA, genomic sequence cloning and overexpression of the ribosomal protein S13 gene in the giant panda (Ailuropoda melanoleuca)”, vol. 10, pp. 121-132, 2011.

The cDNA and the genomic sequence of ribosomal protein S13 (RPS13) of the giant panda (Ailuropoda melanoleuca) was cloned using reverse transcription-polymerase chain reaction (RT-PCR) and touchdown-PCR, respectively. These two sequences were sequenced and analyzed, and the cDNA of the RPS13 gene was overexpressed in Escherichia coli BL21. We compared the nucleotide sequences of the coding region and the amino acid sequences with those of seven other mammalian species retrieved from GenBank.

Subscribe to Sequence analysis