Sequence analysis

Cloning and sequence analysis of the coding sequence of β-actin cDNA from the Chinese alligator and suitable internal reference primers from the β-actin gene

H. N. Zhu, Zhang, S. Z., Zhou, Y. K., Wang, C. L., and Wu, X. B., Cloning and sequence analysis of the coding sequence of β-actin cDNA from the Chinese alligator and suitable internal reference primers from the β-actin gene, vol. 14, pp. 12159-12167, 2015.

β-Actin is an essential component of the cytoskeleton and is stably expressed in various tissues of animals, thus, it is commonly used as an internal reference for gene expression studies. In this study, a 1731-bp fragment of β-actin cDNA from Alligator sinensis was obtained using the homology cloning technique. Sequence analysis showed that this fragment contained the complete coding sequence of the β-actin gene (1128 bp), encoding 375 amino acids.

Establishment of reference sequences of hepatitis B virus genotype C subgenotypes

H. L. Zhu, Wang, C. T., Xia, J. B., Li, X., and Zhang, Z. H., Establishment of reference sequences of hepatitis B virus genotype C subgenotypes, vol. 14, pp. 16521-16534, 2015.

Hepatitis B virus genotype C (HBV/C) has the largest number of subgenotypes (C1-C16) that vary with geography and isolates. HBV/C prevails in Southeast Asia (C1, C5-C16), East Asia (C2), Oceania (C3), and Australia (C4). Suitable reference strains for different subgenotypes could greatly facilitate research into HBV/C, but unfortunately they are scarce. We retrieved 974 HBV/C full-length sequences from the GenBank database and subgenotyped them by phylogenetic analysis.

Cloning and transformation analysis of isoflavone synthase gene into Minshan Trifolium pratense

H. H. Hu, Jing, C. Q., Liu, R., Li, W. D., and Feng, H. G., Cloning and transformation analysis of isoflavone synthase gene into Minshan Trifolium pratense, vol. 14, pp. 9291-9297, 2015.

The aim of this study was to clone the isoflavone synthase (IFS) gene and establish the recombinant Minshan Trifolium pratense. The IFS gene was cloned from the callus of Minshan T. pratense using reverse transcription-polymerase chain reaction. The plant expression vector pRI101-AN-IFS was constructed and introduced into Agrobacterium tumefaciens strain LBA4404, and then screened under cephalosporin. IFS expression was detected by reverse transcription-polymerase chain reaction. The IFS gene was cloned successfully.

Ectopic expression of the BoTFL1-like gene of Bambusa oldhamii delays blossoming in Arabidopsis thaliana and rescues the tfl1 mutant phenotype

H. Y. Zeng, Lu, Y. T., Yang, X. M., Xu, Y. H., and Lin, X. C., Ectopic expression of the BoTFL1-like gene of Bambusa oldhamii delays blossoming in Arabidopsis thaliana and rescues the tfl1 mutant phenotype, vol. 14, pp. 9306-9317, 2015.

TERMINAL FLOWER1 (TFL1) homologous genes play major roles in maintaining vegetative growth and inflorescence meristem characteristics in various plant species; however, to date, the function of the bamboo TFL1 homologous gene has not been described. In this study, a TFL1 homologous gene was isolated from Bambusa oldhamii and designated as BoTFL1-like. Phylogenetic analysis of TFL1 homologous genes revealed that BoTFL1-like shared more than 90% identity with the TFL1 genes of other Gramineae.

Identification and analysis of the TIFY gene family in Gossypium raimondii

D. H. He, Lei, Z. P., Tang, B. S., Xing, H. Y., Zhao, J. X., and Jing, Y. L., Identification and analysis of the TIFY gene family in Gossypium raimondii, vol. 14, pp. 10119-10138, 2015.

The highly conserved TIFY domain is included in the TIFY protein family of transcription factors, which is important in plant development. Here, 28 TIFY family genes were identified in the Gossypium raimondii genome and classified into JAZ (15 genes), ZML (8), PPD (3), and TIFY (2). The normal (TIF[F/Y]XG) motif was dominant in the TIFY family, excluding the ZML subfamily, in which TLSFXG was prevalent. TIFY family genes were unevenly distributed in the G.

Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana

W. Y. Xuan, Zhang, Y., Liu, Z. Q., Feng, D., and Luo, M. Y., Molecular cloning and expression analysis of a novel BCCP subunit gene from Aleurites moluccana, vol. 14, pp. 9922-9931, 2015.

Aleurites moluccana L. is grown as a roadside tree in southern China and the oil content of its seed is higher than other oil plants, such as Jatropha curcas and Camellia oleifera. A. moluccana is considered a promising energy plant because its seed oil could be used to produce biodiesel and bio-jet fuel. In addition, the bark, leaves, and kernels of A. moluccana have various medical and commercial uses. Here, a novel gene coding the biotin carboxyl carrier protein subunit (BCCP) was cloned from A. moluccana L.

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