semen

Comparative study of DNA extraction methodologies from goat sperm and its effects on polymerase chain reaction analysis

E. C. B. Silva, Pelinca, M. A., Acosta, A. C., Silva, D. M. F., Filho, M. A. Gomes, and Guerra, M. M. P., Comparative study of DNA extraction methodologies from goat sperm and its effects on polymerase chain reaction analysis, vol. 13, pp. 6070-6078, 2014.

Successful DNA extraction is indispensable for molecular methods based on polymerase chain reaction (PCR); however, goat sperm DNA extraction is limited. Thus, the aim of this study was to evaluate three methods to extract DNA from goat sperm for use in PCR. Eight goat semen pools were used for DNA extraction by using the DNeasy Blood & Tissue Kit, phenol-chloroform, and Chelex-100 methods.

Relationship between the magnitude of the inbreeding coefficient and milk traits in Holstein and Jersey dairy bull semen used in Brazil

M. P. Soares, Gaya, L. G., Lorentz, L. H., Batistel, F., Rovadoscki, G. A., Ticiani, E., Zabot, V., Di Domenico, Q., Madureira, A. P., and Pértile, S. F. N., Relationship between the magnitude of the inbreeding coefficient and milk traits in Holstein and Jersey dairy bull semen used in Brazil, vol. 10, pp. 1942-1947, 2011.

Artificial insemination has been used to improve production in Brazilian dairy cattle; however, this can lead to problems due to increased inbreeding. To evaluate the effect of the magnitude of inbreeding coefficients on predicted transmitting abilities (PTAs) for milk traits of Holstein and Jersey breeds, data on 392 Holstein and 92 Jersey sires used in Brazil were tabulated. The second-degree polynomial equations and points of maximum or minimal response were estimated to establish the regression equation of the variables as a function of the inbreeding coefficients.

Bovine papillomavirus DNA in milk, blood, urine, semen, and spermatozoa of bovine papillomavirus-infected animals

C. J. Lindsey, Almeida, M. E., Vicari, C. F., Carvalho, C., Yaguiu, A., Freitas, A. C., Beçak, W., and Stocco, R. C., Bovine papillomavirus DNA in milk, blood, urine, semen, and spermatozoa of bovine papillomavirus-infected animals, vol. 8, pp. 310-318, 2009.

Papillomavirus infection in bovines is associated with cutaneous papillomatosis on the hide, udders and other epithelial tissues, as well as in oral respiratory, alimentary and urinary tract mucosa. Bovine papillomavirus (BPV) is also considered the etiological agent of esophageal tumors and the malignant bladder tumors that characterize the clinical condition associated with chronic enzootic hematuria.

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