Sebastiscus marmoratus

Isolation of new polymorphic microsatellite markers from the marbled rockfish Sebastiscus marmoratus

H. W. Deng, Li, Z. B., Dai, G., Yuan, Y., Ning, Y. F., Shangguan, J. B., and Huang, Y. S., “Isolation of new polymorphic microsatellite markers from the marbled rockfish Sebastiscus marmoratus”, vol. 14, pp. 758-762, 2015.

The marbled rockfish, Sebastiscus marmoratus, is an important commercially near-shore fish that inhabits the beach rocky bottom from Japan to the South China Sea. Eleven polymorphic microsatellite loci were developed from S. marmoratus and were used to identify polymorphisms in 30 samples from a wild population. The allele locus number ranged from 2 to 7. Polymorphism data content ranged from 0.032 to 0.751. The observed and expected heterozygosity levels were 0.0333-0.9667 and 0.0328-0.7675, respectively.

Polymorphic microsatellite markers in the false kelpfish Sebastiscus marmoratus: isolation, characterization, and cross-species amplification

H. B. Liu, Liu, S. F., Ye, J. B., Yuan, Y. J., Ding, S. X., and Zhuang, Z. M., “Polymorphic microsatellite markers in the false kelpfish Sebastiscus marmoratus: isolation, characterization, and cross-species amplification”, vol. 13. pp. 134-138, 2014.

A (GT/CA)13-microsatellite-enriched genomic library of the false kelpfish Sebastiscus marmoratus was constructed, and 20 polymorphic microsatellite loci were isolated and characterized. The polymorphisms were investigated in 48 wild individuals from a single population collected from the northern Yellow Sea. The numbers of alleles per locus varied from 4-22 with an average of 9. The observed and expected heterozygosities of each locus ranged from 0.196-0.958 and from 0.487-0.942, with an average of 0.693 and 0.765, respectively.

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