SDS-PAGE

Pericarp polypeptides and SRAP markers associated with fruit quality traits in an interspecific tomato backcross

J. H. Pereira da Costa, Rodríguez, G. R., Pratta, G. R., Picardi, L. A., and Zorzoli, R., Pericarp polypeptides and SRAP markers associated with fruit quality traits in an interspecific tomato backcross, vol. 13, pp. 2539-2547, 2014.

The aim of this study was to detect polypeptides and genomic regions associated with fruit quality traits in a backcross generation using as parent the Argentinean cultivated tomato Caimanta of Solanum lycopersicum and the wild accession LA722 of S. pimpinellifolium. We tested two types of molecular marker: polypeptide profile (at two ripening stages, mature green and red ripe) and SRAP (sequence-related amplified polymorphism). A polypeptide of 45 kDa present in the wild parents at the mature green stage was associated with larger fruit and long shelf life.

Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa

J. J. Cui, Tian, Y., Liu, Y., Huang, H. L., and Jin, Y., Proacrosin activation mechanisms in capacitated and frozen-thawed boar spermatozoa, vol. 13, pp. 9915-9920, 2014.

The main objective of the current study was to explore the different activation mechanisms of capacitation and freeze-thawed spermatozoa. Using SDS-PAGE and Western blotting, the conversion process of boar proacrosin during freeze-thawing and capacitation of spermatozoa was analyzed. The results revealed that capacitated spermatozoa exhibited a greater fluorescence area than that of the freeze-thawed spermatozoa, which were smaller than those of the fresh group.

Molecular characterization of the pathogenic plant fungus Rhizoctonia solani (Ceratobasidiaceae) isolated from Egypt based on protein and PCR-RAPD profiles

M. A. Mahmoud, Al-Sohaibani, S. A., Abdelbacki, A. M. M., Al-Othman, M. R., Aziz, A. R. M. Abd El-, Kasem, K. K., Mikhail, M. S., Sabet, K. K., Omar, M. R., and Hussein, E. M., Molecular characterization of the pathogenic plant fungus Rhizoctonia solani (Ceratobasidiaceae) isolated from Egypt based on protein and PCR-RAPD profiles, vol. 11, pp. 3585-3600, 2012.

Twenty-one isolates of Rhizoctonia solani were categorized into three anastomosis groups consisting of AG-4-HG-I (eight isolates), AG-2-2 (nine isolates) and AG-5 (four isolates). Their pathogenic capacities were tested on cotton cultivar Giza 86. Pre-emergence damping-off varied in response to the different isolates; however, the differences were not significant. Soluble proteins of the fungal isolates were electrophoresed using SDS-PAGE and gel electrophoreses.

Comparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and restricted fragment length polymorphism among fenugreek accessions

E. A. Haliem and Al-Huqail, A. A., Comparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and restricted fragment length polymorphism among fenugreek accessions, vol. 12, pp. 6284-6298, 2013.

Protein and DNA polymorphismswere surveyed among seven accessions of wild fenugreek (Trigonellafoenum-graecum L.) to estimate their genetic diversity and relationships. Samples were obtained from diverse ecogeographical areas in Saudi Arabia and Yemen. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of seed storage protein showed genetic variations among fenugreek germplasms, both quantitatively and qualitatively, generating a total of 168 polypeptide bands with different molecular weights ranging from 4.5 to 300 kDa.

Elucidation of thermotolerance diversity in cotton (Gossypium hirsutum L.) using physio-molecular approaches

R. M. Rana, Khan, S. H., Ali, Z., Khan, A. I., and Khan, I. A., Elucidation of thermotolerance diversity in cotton (Gossypium hirsutum L.) using physio-molecular approaches, vol. 10, pp. 1156-1167, 2011.

Cotton (Gossypium hirsutum) is an important cash crop, but high temperature during its growing season is one of the major factors that limit its productivity. This problem compels plant breeders to breed for heat tolerance, which can help to overcome this challenge. It is very important to make a comprehensive screening of heat-tolerant genotypes so that only the best are chosen. Here we report the combined use of several techniques that can help breeders to screen their germplasm.

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