Sclerotinia sclerotiorum

Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)

R. C. C. Vasconcellos, Lima, T. F. C., Fernandes-Brum, C. N., Chalfun-Júnior, A., Santos, J. B., Vasconcellos, R. C. C., Lima, T. F. C., Fernandes-Brum, C. N., Chalfun-Júnior, A., and Santos, J. B., “Expression and validation of PvPGIP genes for resistance to white mold (Sclerotinia sclerotiorum) in common beans (Phaseolus vulgaris L.)”, vol. 15, p. -, 2016.

The interaction between polygalacturonase-inhibiting proteins (PGIPs), produced by plants, and endopolygalacturonases (PGs), produced by fungi, limits the destructive potential of PGs and can trigger plant defense responses.

White mold resistance-associated quantitative trait loci in the Jalo x Small White common bean population

D. A. Souza, Balestre, M., Pamplona, A. K. A., Leite, M. E., Dias, J. A., Santos, J. B., Souza, D. A., Balestre, M., Pamplona, A. K. A., Leite, M. E., Dias, J. A., and Santos, J. B., “White mold resistance-associated quantitative trait loci in the Jalo x Small White common bean population”, vol. 15, p. -, 2016.

We aimed to identify simple sequence repeat (SSR) markers linked to quantitative trait loci (QTLs) associated with white mold resistance in a segregating population derived from a cross between common bean cultivars Jalo and Small White, in the Southern State of Minas Gerais. Parents were crossed to obtain the F2 generation of 190 plants. From these, F2:3 and F2:4 progenies were obtained for phenotypic evaluation. DNA was extracted from F2 plants and parents for genotyping with SSR primers.

Purification, characterization, and heterologous expression of an antifungal protein from the endophytic Bacillus subtilis strain Em7 and its activity against Sclerotinia sclerotiorum

N. N. Wang, Gao, X. N., Yan, X., Li, Z. P., Kang, Z. S., Huang, L. L., and Han, Q. M., “Purification, characterization, and heterologous expression of an antifungal protein from the endophytic Bacillus subtilis strain Em7 and its activity against Sclerotinia sclerotiorum”, vol. 14, pp. 15488-15504, 2015.

An antifungal protein exhibiting a high activity against Sclero­tinia sclerotiorum in vivo was purified by ammonium sulfate precipitation, hydrophobic chromatography, and gel filtration chromatography from the culture filtrate of the endophytic Bacillus subtilis strain Em7. The protein was characterized as a β-1,3-1,4-glucanase according to amino acid analysis, and showed excellent properties in thermal stability and acid resistance. At the same time, the antifungal protein was cloned and het­erologously expressed in Escherichia coli BL21.

Detection and characterization of carbendazim resistance in Sclerotinia sclerotiorum isolates from oilseed rape in Anhui Province of China

D. Xu, Pan, Y., Zhang, H., Li, X., Dai, Y., Cao, S., and Gao, Z., “Detection and characterization of carbendazim resistance in Sclerotinia sclerotiorum isolates from oilseed rape in Anhui Province of China”, vol. 14, pp. 16627-16638, 2015.

Stem rot caused by Sclerotinia sclerotiorum is a devastating disease of oilseed rape (Brassica napus) in Anhui Province of China. The fungicide carbendazim (methyl benzimidazole-2-yl carbamate; MBC) has been used to control this fungal disease since the 1980s. In the present study, 74 isolates of S. sclerotiorum from 13 regions of Anhui were collected, and the sensitivities of these isolates to MBC were examined to monitor fungicide resistance. We found that 22 of the 74 isolates showed resistance to MBC, indicating that S.

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