DNase concentration assay to obtain DNA-free RNA from sugarcane leaves.
, “DNase concentration assay to obtain DNA-free RNA from sugarcane leaves.”, Genet Mol Res, vol. 15, no. 4, 2016.
Saccharum
Selection of sugar cane families by using BLUP and multi-diverse analyses for planting in the Brazilian savannah
, “Selection of sugar cane families by using BLUP and multi-diverse analyses for planting in the Brazilian savannah”, vol. 13, pp. 1619-1626, 2014.
This study evaluated different strategies to select sugar cane families and obtain clones adapted to the conditions of the Brazilian savannah. Specifically, 7 experiments were conducted, with 10 full sib families, and 2 witnesses in common to all experiments, in each experiment. The plants were grown in random blocks, with witnesses in common (incomplete blocks), and 6 repetitions of each experiment. The data were analyzed through the methodology of mixed patterns, in which the matrices of kinship between the families were identified by the method of restricted maximum likelihood.
Genetic studies of “noble cane” for identification and exploitation of genetic markers
, “Genetic studies of “noble cane” for identification and exploitation of genetic markers”, vol. 9, pp. 1011-1022, 2010.
Forty genotypes (clones) of sugarcane, including elite lines, commercial cultivars of Saccharum officinarum and clones of S. barberi were fingerprinted with 50 SSR markers using a PCR-based marker assay. Nei’s genetic distances for SSR data were determined and relationships between accessions were portrayed graphically in the form of a dendrogram. Genetic distance values ranging from 0.60 to 1.11 were observed among the 40 sugarcane accessions. The shortest genetic distance of 0.60 was seen between genotypes US-804 and US-130.
Abundance and diversity of resistance genes in the sugarcane transcriptome revealed by in silico analysis
, “Abundance and diversity of resistance genes in the sugarcane transcriptome revealed by in silico analysis”, vol. 6, pp. 866-889, 2007.
Resistance genes (R-genes) are responsible for the first interaction of the plant with pathogens being responsible for the activation (or not) of the defense response. Despite their importance and abundance, no tools for their automatic annotation are available yet. The present study analyzed R-genes in the sugarcane expressed sequence tags database which includes 26 libraries of different tissues and development stages comprising 237,954 expressed sequence tags.
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