RT-PCR

Rapamycin enhances IFN-γ and IL-4 production in co-culture of gδ T and dendritic cells from mice with lipopolysaccharide-induced acute lung injury

X. L. Chen, Li, J., Xu, G. G., Li, H. X., Guo, J., Chen, X. L., Li, J., Xu, G. G., Li, H. X., Guo, J., Chen, X. L., Li, J., Xu, G. G., Li, H. X., and Guo, J., Rapamycin enhances IFN-γ and IL-4 production in co-culture of gδ T and dendritic cells from mice with lipopolysaccharide-induced acute lung injury, vol. 15, p. -, 2016.

This study aimed to study the role of rapamycin (RAPA) in modulating the interaction between gδ T cells and dendritic cells (DCs) in a lipopolysaccharide (LPS)-induced acute lung injury mouse model. Mice were injected with LPS to establish the acute lung injury model or LPS + RAPA to assess the role of RAPA in modulating cell interactions. Mice were injected with PBS or RAPA alone as controls. gδ T cells and DCs were isolated from all mice and assessed by flow cytometry and fluorescence microscopy.

Detection of CK19, LUNX, and KS1/4 mRNA expression in the peripheral blood for diagnosis of micrometastases in patients with non-small cell lung cancer and their clinical implications

L. Yan, Yao, Y., Wang, L. H., Wang, M. L., and Fu, X. H., Detection of CK19, LUNX, and KS1/4 mRNA expression in the peripheral blood for diagnosis of micrometastases in patients with non-small cell lung cancer and their clinical implications, vol. 14, pp. 15090-15095, 2015.

The expression of CK19, LUNX, and KS1/4 mRNA biomarkers was detected in the peripheral blood of non-small cell lung cancer (NSCLC) patients to investigate the feasibility of indicating lung cancer micrometastases. Micrometastases were identified in the peripheral blood of 32 NSCLC patients, 15 benign pulmonary disease (BPD) patients, and 10 healthy volunteers by reverse transcriptase-polymerase chain reaction.

Expression profile of the GA733 gene family in colorectal cancer: correlation with clinicopathological parameters

I. Mamaloudis, Zacharoulis, D., Samara, M., Papadopoulos, G., Samara, S., Koukoulis, G., Chatzitheofilou, C., and Kollia, P., Expression profile of the GA733 gene family in colorectal cancer: correlation with clinicopathological parameters, vol. 14, pp. 14772-14781, 2015.

GA733-1/-2/-3 genes have been detected in various types of cancer, although their role has not been fully clarified. GA733-2 and GA733-1 have been correlated with lymph node metastases in laryngeal cancer and liver metastases, respectively. Only a few studies have elucidated the mechanisms regulating GA733-1/-2 expression and their effect on colorectal cancer. Therefore, the expression pattern and the role of the aforementioned molecules in colorectal carcinogenesis were evaluated in this study.

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