RPS14

Giant panda ribosomal protein S14: cDNA, genomic sequence cloning, sequence analysis, and overexpression

G. - F. Wu, Hou, Y. - L., Hou, W. - R., Song, Y., and Zhang, T., Giant panda ribosomal protein S14: cDNA, genomic sequence cloning, sequence analysis, and overexpression, vol. 9, pp. 2004-2015, 2010.

RPS14 is a component of the 40S ribosomal subunit encoded by the RPS14 gene and is required for its maturation. The cDNA and the genomic sequence of RPS14 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively; they were both sequenced and analyzed. The length of the cloned cDNA fragment was 492 bp; it contained an open-reading frame of 456 bp, encoding 151 amino acids. The length of the genomic sequence is 3421 bp; it contains four exons and three introns.

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