RNAi

Effect of lentivirus-mediated RNA interference of APC-Cdh1 expression on spinal cord injury in rats

Y. - H. Qi, Yao, W. - L., Zhang, C. - H., and Guo, Y. - Q., Effect of lentivirus-mediated RNA interference of APC-Cdh1 expression on spinal cord injury in rats, vol. 13, pp. 1366-1372, 2014.

This study investigated cadherin-1 (Cdh1) expression in the sensorimotor cortex of rats after spinal cord injury (SCI). The repairing effect of Cdh1 was evaluated by silencing its expression with lentivirus-mediated RNAi. Twenty male Sprague-Dawley (SD) rats were randomly divided into a normal group and an operation group. Rats of the operation group were given SCI by the Allen method (T10-T11). Cdh1 expression in the sensorimotor cortex was examined by quantitative real-time polymerase chain reaction (PCR) and Western blot analysis.

Efficient delivery of connective tissue growth factor shRNA using PAMAM nanoparticles

Z. J. Huang, Yi, B., Yuan, H., and Yang, G. P., Efficient delivery of connective tissue growth factor shRNA using PAMAM nanoparticles, vol. 13, pp. 6716-6723, 2014.

The aim of this study was to detect the anti-fibrosis activity of connective tissue growth factor (CTGF) small hairpin RNA (shRNA) mediated by polyamidoamine dendrimer nanoparticles in rat myocardial cell lines and myocardium. CTGF shRNAs were constructed from inverted oligonucleotides and a polyamidoamine nanoparticle vector was used to transfer shRNA into H9c2 myocardial cells and spontaneously hypertensive rats.

Knockdown of chimeric glucocerebrosidase by green fluorescent protein-directed small interfering RNA

T. N. Campbell and Choy, F. Y. M., Knockdown of chimeric glucocerebrosidase by green fluorescent protein-directed small interfering RNA, vol. 3, pp. 282-287, 2004.

Gaucher disease, the most common type of lysosomal storage disorder, is characterized by an inherited deficiency of the membrane-associated hydrolase, glucocerebrosidase. Glucocerebrosidase catalyzes the hydrolysis of glucocerebroside to ceramide and glucose, a crucial step in the recycling of membrane sphingolipids. The exorbitant cost of the current treatment standard for Gaucher disease, enzyme replacement therapy, prevents many from receiving treatment.

Subscribe to RNAi