RNA sequencing

De novo assembly and characterization of skin transcriptome using RNAseq in sheep (Ovis aries)

Y. J. Yue, Liu, J. B., Yang, M., Han, J. L., Guo, T. T., Guo, J., Feng, R. L., and Yang, B. H., De novo assembly and characterization of skin transcriptome using RNAseq in sheep (Ovis aries), vol. 14, pp. 1371-1384, 2015.

Wool is produced via synthetic processes of wool follicles, which are embedded in the skin of sheep. The development of new-generation sequencing and RNA sequencing provides new approaches that may elucidate the molecular regulation mechanism of wool follicle development and facilitate enhanced selection for wool traits through gene-assisted selection or targeted gene manipulation. We performed de novo transcriptome sequencing of skin using the Illumina Hiseq 2000 sequencing system in sheep (Ovis aries).

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