RNA interference

Effects of monomethoxypolyethylene glycol-chitosan nanoparticle-mediated dual silencing of livin and survivin genes in prostate cancer PC-3M cells

A. Q. Yang, Wang, P. J., Huang, T., Zhou, W. L., Landman, J., Yang, A. Q., Wang, P. J., Huang, T., Zhou, W. L., Landman, J., Yang, A. Q., Wang, P. J., Huang, T., Zhou, W. L., and Landman, J., “Effects of monomethoxypolyethylene glycol-chitosan nanoparticle-mediated dual silencing of livin and survivin genes in prostate cancer PC-3M cells”, vol. 15, p. -, 2016.

Monomethoxypolyethylene glycol-chitosan (mPEG-CS) nanoparticles were used as interfering RNA carriers to transfect human prostate cancer PC-3M cells to evaluate the effects of livin and survivin gene silencing on the proliferation and apoptosis. mPEG-CS nanoparticles with sizes of approximately 60 nm were first synthesized by ionic crosslinking. Through electrostatic adsorption, mPEG-CS-livin short hairpin RNA (shRNA), mPEG-CS-survivin shRNA, and mPEG-CS-(livin shRNA + survivin shRNA) nanoparticles were then prepared to transfect PC-3M cells.

Segment-specific targeting via RNA interference mediates down-regulation of OPN expression in hepatocellular carcinoma cells

F. Lin, Huang, C. M., Cao, J., Pei, Z. H., Gu, W. L., Fan, S. F., Li, K. P., and Lin, C. M., “Segment-specific targeting via RNA interference mediates down-regulation of OPN expression in hepatocellular carcinoma cells”, vol. 14, pp. 14440-14447, 2015.

Osteopontin (OPN) plays an important role in the metastasis and recurrence of tumors after resection of hepatocellular carcinoma (HCC). In this study, the down-regulation effect on OPN expression in HCC cells of RNA interference (RNAi) molecules designed to target different segments of OPN was investigated to identify a more effective site for OPN knockdown. Specific small interfering RNAs (siRNAs A, B, and C) of OPN were synthesized and transfected into an HCC cell line (HEP-G2; representing the OPNi-A, OPNi-B, and OPNi-C groups).

Influence of suppression of CapG gene expression by siRNA on the growth and metastasis of human prostate cancer cells

B. K. Li, Guo, K., Li, C. Y., Li, H. L., Zhao, P. P., Chen, K., and Liu, C. X., “Influence of suppression of CapG gene expression by siRNA on the growth and metastasis of human prostate cancer cells”, vol. 14, pp. 15769-15778, 2015.

This study investigated CapG gene expression in prostate cancer cell lines; in addition, we explored the effects of CapG suppression on DU145 cell growth, and the underlying mechanism with which CapG affects DU145 cell growth and invasiveness. The expression of CapG and 18 related genes in DU145 cells was analyzed by flow cytometry, quantitative polymerase chain reaction (qPCR), CCK8 assay, western blot, and the trans-well assay. DU145 cells were transfected with designed small interfering RNA (siRNA). CapG expression was quantified by qPCR and western blot.

Effect of siRNA targeting HER2/neu on the proliferation and viability of prostate cancer PC-3M cells

C. Y. Liu, Xu, P. C., Chen, D. G., Fan, X. H., Li, M. Q., Yang, X., and Xu, Y. P., “Effect of siRNA targeting HER2/neu on the proliferation and viability of prostate cancer PC-3M cells”, vol. 14, pp. 17145-17153, 2015.

The aim of this study was to investigate the effect of a small interfering RNA (siRNA) targeting human epidermal growth factor receptor 2 (HER2/neu) on the proliferation and viability of prostate cancer PC-3M cells. Chemically synthesized siRNA targeting HER2/neu was transfected into PC-3M cells by using liposomes, and cells transfected with empty liposomes, a negative siRNA sequence, or nothing (untransfected) were used as controls. mRNA and protein levels of HER2/neu were detected using reverse transcription-polymerase chain reaction and western blot, respectively.

N-ethylmaleimide-sensitive factor siRNA improves cardiac function following myocardial infarction in rats

Y. Zhou, Liu, Y., Yang, S. X., and Wang, Z., “N-ethylmaleimide-sensitive factor siRNA improves cardiac function following myocardial infarction in rats”, vol. 14, pp. 9478-9485, 2015.

This study examined the effects of N-ethylmaleimide-sensitive factor (NSF) small interfering RNA (siRNA) on cardiac function following myocardial infarction (MI) in rats. Thirty-six adult Sprague Dawley rats were randomly divided into three equivalent groups. An acute MI model was established by ligating the anterior descending branch of the left coronary artery and confirmed by electrocardiogram. Recombinant NSF-siRNA adenovirus (experimental), negative adenovirus (control), and normal saline were injected near the infarcted area of the left ventricle in each respective group.

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