Reverse transcription-polymerase chain reaction

14-3-3 Gene expression in regenerating rat liver after 2/3 partial hepatectomy

D. M. Xue, Guo, X. Q., Chen, R., Niu, Z. P., and Xu, C. S., 14-3-3 Gene expression in regenerating rat liver after 2/3 partial hepatectomy, vol. 14, pp. 2023-2030, 2015.

14-3-3 Proteins are a ubiquitous family of molecules that participate in protein kinase signaling pathways in all eukaryotic cells. Functioning as phosphoserine/phosphothreonine-binding modules, 14-3-3 proteins participate in the phosphorylation-dependent protein-protein interactions that control progression through the cell cycle, initiation and maintenance of DNA damage checkpoints, activation of MAP kinases, prevention of apoptosis, and coordination of integrin signaling and cytoskeletal dynamics.

Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia

C. Gutiérrez-Vergara, Quintero, J., Duarte, J. F., Suescún, J. P., and López-Herrera, A., Detection of hepatitis E virus genome in pig livers in Antioquia, Colombia, vol. 14, pp. 2890-2899, 2015.

Hepatitis E is a form of endemic acute hepatitis found in humans in many countries worldwide and is caused by the hepatitis E Virus (HEV). Detection of HEV in pigs indicates that they may be carriers, possibly through zoonosis. The prevalence of HEV in pigs in Colombia is unknown. Studies in the US found that 11% of pig livers sold in grocery stores are contaminated with HEV. It is also known that HEV can be inactivated when cooked, as it is labile to high temperatures. The aim of this study was to determine HEV contamination in pig livers sold in Medellín, Antioquia.

Transcriptional activity of an ovarian-specific promoter from rat in dairy goat granulosa cells

H. - Y. Liu, Yang, M. - M., Cui, Y. - H., Ma, T., Liu, X. - Q., Bai, L., Xiao, W. - P., Zhao, H. - B., Peng, J. - Y., Hu, X. - B., and Cao, B. - Y., Transcriptional activity of an ovarian-specific promoter from rat in dairy goat granulosa cells, vol. 12, pp. 127-135, 2013.

Ovarian-specific promoter 1 (OSP-1) is a retrovirus-like element isolated from the complementary DNA library of rat that has been thought to be specifically expressed in ovary. To exploit this promoter in dairy goat ovary granulosa cells (GCs), OSP-1 from rat was used to construct the reporter vector pOSP-1-EGFP, in which egfp coding for enhanced green fluorescent protein (EGFP) was used as a reporter to examine the activity of OSP-1 in GCs. EGFP was successfully expressed in dairy goat GCs transfected with pOSP-1-EGFP.

Identification of PML/RARα fusion gene transcripts that showed no t(15;17) with conventional karyotyping and fluorescent in situ hybridization

A. Choughule, Polampalli, S., Amre, P., Shinde, S., Banavali, S., Prabhash, K., Nair, R., Subramanian, P. G., Gujral, S., and Parikh, P. M., Identification of PML/RARα fusion gene transcripts that showed no t(15;17) with conventional karyotyping and fluorescent in situ hybridization, vol. 8, pp. 1-7, 2009.

Acute promyelocytic leukemia (APL) is characterized by a reciprocal translocation, t(15;17)(q22;q11-21), resulting in the fusion of the promyelocytic leukemia (PML) and retinoic acid receptor alpha (RARα) genes. Using conventional cytogenetic methods, these translocations are normally detected in about 70-90% of patients; most negative results are due to technical problems or cryptic variants.

A rapid and efficient method for isolation of total RNA from Euglena gracilis (Euglenoidea)

D. González-Mendoza, Morales-Trejo, A., and Brito-Vera, H., A rapid and efficient method for isolation of total RNA from Euglena gracilis (Euglenoidea), vol. 8, pp. 482-486, 2009.

RNA isolation is essential to the study of gene expression at the molecular level. However, it is difficult to isolate RNA from organisms that contain large amounts of polysaccharides or other compounds that bind or coprecipitate with RNA, such as the unicellular protist Euglena gracilis. Currently, there is no commercial kit available that is specific for the isolation of high-quality RNA from this organism. Since it contains large amount of polysaccharides, the common protocols for RNA isolation usually result in poor yields when applied to E. gracilis.

Subscribe to Reverse transcription-polymerase chain reaction