Reverse dot hybridization

An improved reverse dot hybridization for simple and rapid detection of adefovir dipivoxil-resistant hepatitis B virus

Y. Hu, Zhang, W. L., Xie, S. L., Zhao, Y., Hu, J. L., Cai, X. F., Lai, G. Q., and Huang, A. L., An improved reverse dot hybridization for simple and rapid detection of adefovir dipivoxil-resistant hepatitis B virus, vol. 11, pp. 53-60, 2012.

Early detection of adefovir dipivoxil-resistant mutants during long-term treatment of chronic hepatitis B virus (HBV) infection with this drug is of great clinical importance. We developed an improved reverse dot hybridization test for simple and rapid detection of the rtA181V/T and rtN236T mutations associated with adefovir dipivoxil resistance in chronic hepatitis B patients. Probes were designed for genotypes B, C, and D of this resistance characteristic; a total of 70 clinical samples were analyzed with this improved reverse dot hybridization assay.

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