Restriction fragment length polymorphism

No association between FGD1 gene polymorphisms and intellectual developmental disability in the Qinba mountain area

J. L. Li, Li, Y. J., Zhang, K. J., Lan, L., Shi, J. G., Yang, X., Zhang, M. J., Zhang, F. C., and Gao, X. C., No association between FGD1 gene polymorphisms and intellectual developmental disability in the Qinba mountain area, vol. 13, pp. 127-133, 2014.

FGD1 encoding a guanine nucleotide exchange factor, specifically activates Rho GTPase cell division cycle 42 (Cdc42). Dysfunction of FGD1 causes Aarskog-Scott syndrome (MIM #305400), an X-linked disorder that may affect bone and intellectual development. However, the relationship between FGD1 and intellectual developmental disorders (IDD) remains unclear. The purpose of this study was to investigate the genetic association between the FGD1 polymorphism and IDD.

Genomic analysis of the interleukin-1β-511 and interleukin-6-174 gene polymorphisms in Turkish patients with epilepsy

İ. Gök, Esen, V., and H. Ozlece, K., Genomic analysis of the interleukin-1β-511 and interleukin-6-174 gene polymorphisms in Turkish patients with epilepsy, vol. 13, pp. 8552-8560, 2014.

In this study, we examined the frequency of polymorphisms in the interleukin (IL) genes IL-1β-511 and IL-6-174 in patients with epilepsy as well as a control group in Kars, Turkey. A total of 100 patients diagnosed with epilepsy and 100 nonepileptic subjects as a control group were examined. Peripheral blood samples were acquired from patients and control subjects for DNA extraction. The target region was amplified using polymerase chain reaction and digested using the restriction enzymes SfaNI and AvaI.

Roles of beta2-adrenergic receptor gene polymorphisms in a Turkish population with obstructive sleep apnea syndrome or obesity

İ. Gök, Çelebi, İ., Hüseyinoğlu, N., and Özic, C., Roles of beta2-adrenergic receptor gene polymorphisms in a Turkish population with obstructive sleep apnea syndrome or obesity, vol. 13, pp. 8511-8518, 2014.

We determined the distribution of the Arg16Gly and Gln27Glu polymorphisms of the beta-2 adrenergic receptor gene (ADRB2) in patients with obstructive sleep apnea syndrome as well as a control group in Northeastern Turkey. A total of 52 patients diagnosed with obstructive sleep apnea in a sleep laboratory and 78 control subjects were examined. Peripheral blood samples were taken from patients diagnosed with obstructive sleep apnea by polysomnography. DNA was extracted from blood samples and amplified using polymerase chain reaction.

Association between CYP1A1m1 gene polymorphism and primary open-angle glaucoma

N. B. Costa, Silva, C. T. X., Frare, A. B., Silva, R. E., and Moura, K. K. V. O., Association between CYP1A1m1 gene polymorphism and primary open-angle glaucoma, vol. 13, pp. 10382-10389, 2014.

The CYP1A1 gene is related to the generation of secondary metabolites that are capable of inducing DNA damage. The CYP1A1m1 polymorphism has been examined in many studies, and is located in a region near loci that have been linked to glaucoma, including the locus GLC1I. As a result, this polymorphism has been related to several diseases that are influenced by exposure to xenobiotic as well as primary open-angle glaucoma.

BDNF and DARPP-32 genes are not risk factors for schizophrenia in the Malay population

H. C. Loh, Tang, P. Y., Tee, S. F., Chow, T. J., Cheah, Y. C., and Singh, S. S. J., BDNF and DARPP-32 genes are not risk factors for schizophrenia in the Malay population, vol. 11, pp. 725-730, 2012.

A number of studies have pointed to the association of BDNF (brain-derived neurotrophic factor) and DARPP-32 (dopamine- and cAMP-regulated phosphoprotein, 32 kDa) with schizophrenia. The purpose of this study was to determine whether these two genes are involved in the pathogenesis of schizophrenia in the Malay population. Two single nucleotide polymorphisms Val66Met of BDNF, -2036C>G and g.1238delG of DARPP-32 were genotyped in the Malay population in 200 patients with schizophrenia and 256 healthy controls.

No evidence for association between DRD3 and COMT with schizophrenia in a Malay population

S. F. Tee, Tang, P. Y., and Loh, H. C., No evidence for association between DRD3 and COMT with schizophrenia in a Malay population, vol. 10, pp. 1850-1855, 2011.

Molecular components of the dopamine D3 receptor (DRD3) may play an important role in the pathophysiology of schizophrenia. Previous studies have demonstrated an association between DRD3 Ser9Gly and cathechol-o-methyltransferase (COMT, SNP = rs165656) polymorphisms and schizophrenia but the results were inconclusive. We investigated this apparent association between Ser9Gly (A/G) polymorphism and an intronic SNP (dbSNP or rs165656) in 261 Malay patients diagnosed with schizophrenia and 216 controls, using PCR-RFLP.

Effects of polymorphisms of LHR and FSHR genes on sexual precocity in a Bos taurus x Bos indicusbeef composite population

E. P. Marson, Ferraz, J. B. S., Meirelles, F. V., Balieiro, J. C. C., and Eler, J. P., Effects of polymorphisms of LHR and FSHR genes on sexual precocity in a Bos taurus x Bos indicusbeef composite population, vol. 7, pp. 243-251, 2008.

The purpose of the present research was to investigate the effects of polymorphisms of luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) genes, evaluated by polymerase chain reaction-restriction fragment length polymorphism in European-Zebu composite beef heifers from six different breed compositions. The polymorphism site analysis from digestion with HhaI and AluI restriction endonucleases allowed the genotype identification for LHR (TT, CT and CC) and FSHR (GG, CG and CC) genes.

Obese gene polymorphism in Pietrain and large white pigs after a divergent selection

A. C. P. Silveira, Antunes, R. C., Almeida, J. F., Braga, T. F., Freitas, P. F. A., César, A. S. M., César, A. S. M., and Guimarães, E. C., Obese gene polymorphism in Pietrain and large white pigs after a divergent selection, vol. 7, pp. 1217-1222, 2008.

The aim of the present study was to identify polymorphisms in the leptin gene of 112 pigs and compare the maternal and paternal lineage of Pietrain and Large White breeds that underwent a divergent selection for over 30 years. DNA samples extracted from the blood of these animals were amplified by polymerase chain reaction and genotyped by restriction fragment length polymorphism using the restriction enzyme HinfI. The data were statistically analyzed by the chi-square test.

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