A novel SCAR marker for detecting Psathyrostachys huashanica Keng chromatin introduced in wheat
“A novel SCAR marker for detecting Psathyrostachys huashanica Keng chromatin introduced in wheat”, vol. 12, pp. 4797-4806, 2013.
, In this study, we cloned and sequenced a 938-base pair polymorphic band, pHs27, in the tightly linked random amplified polymorphic DNA marker OPU10 and converted it into a sequence-characterized amplified region (SCAR) marker referred to as RHS141, which was specific for the Ns genome of Psathyrostachys huashanica. A GenBank basic local alignment search tool search showed that the sequence of pHs27 had no primary sequence homology with known sequences, and Southern blotting confirmed this result.