Reference genes

Reference gene selection for gene expression studies in lily using quantitative real-time PCR

M. F. Zhang, Liu, Q., Jia, G. X., Zhang, M. F., Liu, Q., and Jia, G. X., “Reference gene selection for gene expression studies in lily using quantitative real-time PCR”, vol. 15, p. -, 2016.

Quantitative real-time polymerase chain reaction (qRT-PCR) is an important technology used to analyze gene-expression levels. Reference genes, which are assumed to be expressed consistently across various developmental stages and in different tissues, were selected for expression level analysis. Using digital gene expression technology, we selected nine reference genes (18S, EF, CYCOL, SAND, GAPDH, ACTIN, BHLH, TIP, and Clathrin) as candidate reference genes for further study.

Selection of reference genes in canine uterine tissues

M. Du, Wang, X., Yue, Y. W., Zhou, P. Y., Yao, W., Li, X., Ding, X. B., Liu, X. F., Guo, H., Ma, W. Z., Du, M., Wang, X., Yue, Y. W., Zhou, P. Y., Yao, W., Li, X., Ding, X. B., Liu, X. F., Guo, H., and Ma, W. Z., “Selection of reference genes in canine uterine tissues”, vol. 15, p. -, 2016.

Real-time quantitative polymerase chain reaction (RT-qPCR) is usually employed in gene expression studies in veterinary research, including in studies on canine pyometra. Canine pyometra is a common clinical disease in bitches. When using RT-qPCR, internal standards, such as reference genes, are necessary to investigate relative gene expression by quantitative measurements of mRNA levels. The aim of this study was to evaluate the stability of reference genes and select reference genes suitable for canine pyometra studies.