Expression of recombinant human anti-TNF-α scFv-Fc in Arabidopsis thaliana seeds
“Expression of recombinant human anti-TNF-α scFv-Fc in Arabidopsis thaliana seeds”, vol. 15, p. -, 2016.
, Recombinant human anti-tumor necrosis factor (TNF)-α scFv-Fc was expressed in TKO mutant Arabidopsis thaliana seeds using plant-specific codons. Immunoblotting using a human IgG1 antibody detected the expression of anti-TNF-α proteins in plants. Results from qRT-PCR analysis demonstrated that the time of harvest significantly affected the protein yield and quality. Our results indicate that the Phaseolus vulgaris β-phaseolin promoter directed anti-TNF-α scFv-Fc expression in A. thaliana seeds, with a maximum yield obtained at 20-days of development.