Real-time RT-PCR

Cloning and characterization of a β-amyrin synthase gene from the medicinal tree Aralia elata (Araliaceae)

Y. Wu, Zou, H. D., Cheng, H., Zhao, C. Y., Sun, L. F., Su, S. Z., Li, S. P., and Yuan, Y. P., Cloning and characterization of a β-amyrin synthase gene from the medicinal tree Aralia elata (Araliaceae), vol. 11, pp. 2301-2314, 2012.

Aralia elata is an important medicinal plant in China; it produces large amounts of oleanane type triterpene saponins. A full-length cDNA encoding β-amyrin synthase (designated as AeAS) was isolated from young leaves of A. elata by reverse transcription-PCR. The full-length cDNA of AeAS was found to have a 2292-bp open reading frame, encoding a protein with 763 amino acid residues. The deduced amino acid sequence of AeAS showed the highest identity (97%) to Panax ginseng β-amyrin synthase.

Cloning and characterization of thioredoxin h in the three-pistil line of common wheat

Z. J. Yang, Peng, Z. S., Wu, K., and Wei, S. H., Cloning and characterization of thioredoxin h in the three-pistil line of common wheat, vol. 12, pp. 2688-2701, 2013.

Thioredoxin h (Trxh) is a ubiquitous protein that reduces disulfides in target proteins, and is itself reduced by NADPH-thioredoxin reductase. In the current study, the complementary DNA sequence and the genomic sequence of the three-pistil (TP) line of common wheat (Triticum aestivum L.) were obtained from spikes through reverse transcription-polymerase chain reaction (RT-PCR) and touchdown-PCR. Sequence alignment of amino acids of TPTrxh then allowed for predictions of its physicochemical properties, secondary structures, tertiary structures, and functional domains.

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