Real-time polymerase chain reaction
SHORT-COMMUNICATION Evaluation of perfused bovine udder for gene expression studies in dairy cows.
SHORT-COMMUNICATION Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
Differential expression of Toll-like receptors in goat dominant and nondominant follicles.
Biological correlation between glucose transporters, Ki-67 and 2-deoxy-2-[18F]-fluoro-D-glucose uptake in diffuse large B-cell lymphoma and natural killer/T-cell lymphoma
The purpose of this study was to investigate the association between cellular 2-deoxy-2-[18F]-fluoro-D-glucose (18F-FDG) uptake and the expression of several subtypes of glucose transporters (GLUT) and Ki-67 in diffuse large B-cell lymphoma (DLBCL) and natural killer (NK)/T-cell lymphoma (NKTCL). Cell lines were histologically determined to be DLBCL (Raji cells) and NKTCL (Daudi cells), and uptake after pretreatment with 18F-FDG was determined.
Adaption of SYBR Green-based reagent kit for real-time PCR quantitation of GC-rich DNA
In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing the fluorescent dye SYBR Green. However, these ready-made kits may not be suitable for amplifying DNA samples with a high GC content (>70%).