Real-time polymerase chain reaction

Biological correlation between glucose transporters, Ki-67 and 2-deoxy-2-[18F]-fluoro-D-glucose uptake in diffuse large B-cell lymphoma and natural killer/T-cell lymphoma

Y. M. Liu, Zhai, X. M., Wu, Y. W., Liu, Y. M., Zhai, X. M., and Wu, Y. W., Biological correlation between glucose transporters, Ki-67 and 2-deoxy-2-[18F]-fluoro-D-glucose uptake in diffuse large B-cell lymphoma and natural killer/T-cell lymphoma, vol. 15, p. -, 2016.

The purpose of this study was to investigate the association between cellular 2-deoxy-2-[18F]-fluoro-D-glucose (18F-FDG) uptake and the expression of several subtypes of glucose transporters (GLUT) and Ki-67 in diffuse large B-cell lymphoma (DLBCL) and natural killer (NK)/T-cell lymphoma (NKTCL). Cell lines were histologically determined to be DLBCL (Raji cells) and NKTCL (Daudi cells), and uptake after pretreatment with 18F-FDG was determined.

Adaption of SYBR Green-based reagent kit for real-time PCR quantitation of GC-rich DNA

G. J. Chang, Seyfert, H. M., and Shen, X. Z., Adaption of SYBR Green-based reagent kit for real-time PCR quantitation of GC-rich DNA, vol. 14, pp. 8509-8515, 2015.

In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing the fluorescent dye SYBR Green. However, these ready-made kits may not be suitable for amplifying DNA samples with a high GC content (>70%).

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