Real-time PCR

Transcription factors expressed in soybean roots under drought stress

S. S. Pereira, Guimarães, F. C. M., Carvalho, J. F. C., Stolf-Moreira, R., Oliveira, M. C. N., Rolla, A. A. P., Farias, J. R. B., Neumaier, N., and Nepomuceno, A. L., “Transcription factors expressed in soybean roots under drought stress”, vol. 10, pp. 3689-3701, 2011.

To gain insight into stress-responsive gene regulation in soybean plants, we identified consensus sequences that could categorize the transcription factors MYBJ7, BZIP50, C2H2, and NAC2 as members of the gene families myb, bzip, c2h2, and nac, respectively. We also investigated the evolutionary relationship of these transcription factors and analyzed their expression levels under drought stress.

Expression of Rap1GAP in human myeloid disease following microarray selection

X. Qi, Chen, Z., Qian, J., Cen, J., and Gu, M., “Expression of Rap1GAP in human myeloid disease following microarray selection”, vol. 7, pp. 379-387, 2008.

To find the underlying causes of primary myelodysplastic syndrome (MDS), the gene expression profiling of both CD34+ cells and bone marrow mononuclear cells from MDS patients was performed using oligonucleotide microarray and cDNA microarrays, respectively. Several candidate genes which were differentially expressed in MDS patients versus normal controls were selected and confirmed in expanding samples by quantitative real-time reverse transcription-polymerase chain reaction after clustering and bioinformatics analysis.

Less DmtDNA4977 than normal in various types of tumors suggests that cancer cells are essentially free of this mutation

M. Angela C. Dani, Dani, S. U., Lima, S. P. G., Martinez, A., Rossi, B. M., Soares, F., Zago, M. A., and Simpson, A. J. G., “Less DmtDNA4977 than normal in various types of tumors suggests that cancer cells are essentially free of this mutation”, vol. 3, pp. 395-409, 2004.

Levels of mtDNA4977 deletions (DmtDNA4977) have been found to be lower in tumors than in adjacent non-tumoral tissues. In 87 cancer patients, DmtDNA4977 was detected by multiplex polymerase chain reaction (PCR) amplification in 43 (49%) of the tumors and in 74 (85%) of the samples of non-tumoral tissues that were adjacent to the tumors.

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