Rat

Construction of recombinant adenovirus Ad-rat PLCg2-shRNA and successful suppression of PLCg2 expression in BRL-3A cells

X. G. Chen, Lv, Q. X., Zhou, X. Q., Chen, X. G., Lv, Q. X., and Zhou, X. Q., “Construction of recombinant adenovirus Ad-rat PLCg2-shRNA and successful suppression of PLCg2 expression in BRL-3A cells”, vol. 15, p. -, 2016.

Phospholipase Cg2 (PLCg2) induces apoptosis of immune and tumor cells; however, it remains unclear whether PLCg2 promotes hepatocyte apoptosis during liver regeneration (LR). Therefore, to establish a framework for further exploring the function of PLCg2, we generated recombinant adenoviruses carrying a template encoding short hairpin (sh)-RNA targeting PLCg2 (Ad-PLCg2-shRNA), which were used to silence the expression of PLCg2 in BRL-3A cells. First, three pairs of PLCg2-shRNAs were designed, synthesized, and cloned into a shuttle vector, pHBAd-U6-GFP, after annealing.

Application of retrograde dissection method for isolation of bone marrow cells from rat femurs and tibiae

C. M. Li, Fu, B. M., Zhang, L. C., Tang, B., Zhu, L., Zhao, Y., Zhang, J., Li, C. M., Fu, B. M., Zhang, L. C., Tang, B., Zhu, L., Zhao, Y., and Zhang, J., “Application of retrograde dissection method for isolation of bone marrow cells from rat femurs and tibiae”, vol. 15, p. -, 2016.

Currently, there is no practical and efficient method for the isolation of bone marrow cells (BMCs) from rat femurs and tibiae. Here, we attempted to develop a rapid, simple, effective, and non-contaminating method for the isolation of BMCs from rat femurs and tibiae. Rat femurs and tibiae were dissected from the ankle to the hip joint; subsequently, a three-step “locate-slide-twist” procedure was performed using scissors and forceps to remove the femurs and tibiae completely, from the surrounding musculature. The bones were flushed with phosphate-buffered saline to harvest BMCs.

Rapid and sensitive LC-MS/MS method for the determination of auraptene in rat plasma and its application in a pharmacokinetic and bioavailability study in rats

X. D. Ye, Ouyang, H., Zhong, L. Y., Li, T. E., Rao, X. Y., Feng, Y. L., Yang, W. L., Ye, X. D., Ouyang, H., Zhong, L. Y., Li, T. E., Rao, X. Y., Feng, Y. L., and Yang, W. L., “Rapid and sensitive LC-MS/MS method for the determination of auraptene in rat plasma and its application in a pharmacokinetic and bioavailability study in rats”, vol. 15, p. -, 2016.

A simple, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of auraptene, a constituent isolated from Fructus aurantii with potential to combat Alzheimer’s disease, in rat plasma. Rat plasma samples were pretreated by protein precipitation with methanol. The analytes were separated by a Waters Sun Fire C18 column (50 mm x 2 mm, 5 μm) and eluted with 1:1000 methanol and formic acid/water (v/v) mobile phase with a flow rate of 0.5 mL/min.