Rat

Application of retrograde dissection method for isolation of bone marrow cells from rat femurs and tibiae

C. M. Li, Fu, B. M., Zhang, L. C., Tang, B., Zhu, L., Zhao, Y., Zhang, J., Li, C. M., Fu, B. M., Zhang, L. C., Tang, B., Zhu, L., Zhao, Y., and Zhang, J., Application of retrograde dissection method for isolation of bone marrow cells from rat femurs and tibiae, vol. 15, p. -, 2016.

Currently, there is no practical and efficient method for the isolation of bone marrow cells (BMCs) from rat femurs and tibiae. Here, we attempted to develop a rapid, simple, effective, and non-contaminating method for the isolation of BMCs from rat femurs and tibiae. Rat femurs and tibiae were dissected from the ankle to the hip joint; subsequently, a three-step “locate-slide-twist” procedure was performed using scissors and forceps to remove the femurs and tibiae completely, from the surrounding musculature. The bones were flushed with phosphate-buffered saline to harvest BMCs.

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