RAPD-PCR

Molecular characterization of the pathogenic plant fungus Rhizoctonia solani (Ceratobasidiaceae) isolated from Egypt based on protein and PCR-RAPD profiles

M. A. Mahmoud, Al-Sohaibani, S. A., Abdelbacki, A. M. M., Al-Othman, M. R., Aziz, A. R. M. Abd El-, Kasem, K. K., Mikhail, M. S., Sabet, K. K., Omar, M. R., and Hussein, E. M., Molecular characterization of the pathogenic plant fungus Rhizoctonia solani (Ceratobasidiaceae) isolated from Egypt based on protein and PCR-RAPD profiles, vol. 11, pp. 3585-3600, 2012.

Twenty-one isolates of Rhizoctonia solani were categorized into three anastomosis groups consisting of AG-4-HG-I (eight isolates), AG-2-2 (nine isolates) and AG-5 (four isolates). Their pathogenic capacities were tested on cotton cultivar Giza 86. Pre-emergence damping-off varied in response to the different isolates; however, the differences were not significant. Soluble proteins of the fungal isolates were electrophoresed using SDS-PAGE and gel electrophoreses.

Comparison of winter and summer canola (Brassica napus) genotypes in Turkey

E. Maltas and Vural, H. C., Comparison of winter and summer canola (Brassica napus) genotypes in Turkey, vol. 12, pp. 408-415, 2013.

We examined genetic relationships between canola (Brassica napus) genotypes cultivated in winter and spring in Turkey. Genomic DNA was isolated from the seeds by two modified CTAB protocols: EZ1 nucleic acid isolation method and a commercial kit (Dneasy Plant Mini Kit, Qiagen). Diversity and genetic relationships in the genotypes were analyzed with RAPD markers; 156 reliable bands were found for both genotypes, of which 24% were polymorphic. Fifteen primers gave at least one consistent polymorphic band.

Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis

A. L. D. Oliveira, Da Silva, D., Manzano, B. C., Abdel-Hamid, A. Z., Marcelino, M. Y., Zanotti-Magalhães, E. M., Zanotti-Magalhães, E. M., Magalhães, L. A., and Ribeiro-Paes, J. T., Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis, vol. 9, pp. 1450-1459, 2010.

Despite the implementation control programs, schistosomiasis continues to spread throughout the world. Among modern control strategies, vector control is currently being emphasized. Within this context, analysis of the genetic variability of intermediate host snails (Biomphalaria spp) is important because it allows identification of specific sequences of the genome of this mollusk related to susceptibility/resistance to Schistosoma mansoni infection.

Genetic polymorphism, molecular characterization and relatedness of Macrobrachium species (Palaemonidae) based on RAPD-PCR

A. L. Guerra, Lima, A. V. B., Taddei, F. G., and Castiglioni, L., Genetic polymorphism, molecular characterization and relatedness of Macrobrachium species (Palaemonidae) based on RAPD-PCR, vol. 9, pp. 2317-2327, 2010.

The prawn genus Macrobrachium belongs to the family Palaemonidae. Its species are widely distributed in lakes, reservoirs, floodplains, and rivers in tropical and subtropical regions of South America. Globally, the genus Macrobrachium includes nearly 210 known species, many of which have economic and ecological importance. We analyzed three species of this genus (M. jelskii, M. amazonicum and M.

Schistosome/mollusk: genetic compatibility

A. L. D. Oliveira, Da Silva, D., Zanotti-Magalhães, E. M., Zanotti-Magalhães, E. M., Abdel-Hamid, A. Z., and Ribeiro-Paes, J. T., Schistosome/mollusk: genetic compatibility, vol. 7, pp. 518-526, 2008.

Schistosomiasis remains one of the most prevalent parasitic infections and has significant economic and public health consequences in many developing countries. Economic development and improvement in standard of living in these countries are dependent on the elimination of this odious disease.

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