Random amplified polymorphic DNA markers

Genetic diversity in somatic mutants of grape (Vitis vinifera) cultivar Italia based on random amplified polymorphic DNA

S. H. Z. Maia, Mangolin, C. A., Collet, S. A. O., and Machado, M. F. P. S., Genetic diversity in somatic mutants of grape (Vitis vinifera) cultivar Italia based on random amplified polymorphic DNA, vol. 8, pp. 28-38, 2009.

Random amplified polymorphic DNA (RAPD) markers were used to detect polymorphism and to examine relationships among four table grape clones from northwestern Paraná, in southern Brazil. The 10 primers used for RAPD fingerprints generated 126 reproducible fragments, of which 63, 68, 76, and 72 were polymorphic in cultivars Italia, Rubi, Benitaka, and Brasil, respectively. Among the primers, OPP-08 generated the highest number of fragments, whereas OPE-15 was the most efficient for discriminating polymorphic fragments.

Heirloom tomato gene bank: assessing genetic divergence based on morphological, agronomic and molecular data using a Ward-modified location model

L. S. A. Gonçalves, Rodrigues, R., Júnior, A. Tdo Amaral, Karasawa, M., and Sudré, C. P., Heirloom tomato gene bank: assessing genetic divergence based on morphological, agronomic and molecular data using a Ward-modified location model, vol. 8, pp. 364-374, 2009.

Accessions in gene banks need to be characterized and evaluated to determine their genetic diversity. We made a joint diversity analysis of the tomato gene bank of the Universidade Estadual do Norte Fluminense Darcy Ribeiro in Rio de Janeiro state, using the Ward-modified location model. Forty Solanum lycopersicum accessions were characterized and evaluated for 22 morphoagronomic descriptors and 131 random amplified polymorphic DNA markers. Based on the pseudo-F and pseudo-t2 criteria, the optimal number of groups was established as five.

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