Random amplified polymorphic DNA

Distribution of Candida albicans in the oral cavity of children aged 3-5 years of Uygur and Han nationality and their genotype in caries-active groups

N. Wu, Lin, J., Wu, L., and Zhao, J., Distribution of Candida albicans in the oral cavity of children aged 3-5 years of Uygur and Han nationality and their genotype in caries-active groups, vol. 14, pp. 748-757, 2015.

We analyzed the distribution of Candida albicans in the oral cavity of 3-5-year-old children of Uygur and Han nationalities as well as their genotypes in caries-active groups in the Urumqi municipality. CHROMagar Candida was separately cultivated, and we identified 359 Uygur and Han children aged 3-5 years. We randomly selected 20 Han children and 20 Uygur children for this study. We chose a bacterial strain for polymerase chain reaction (PCR) 25S rDNA genotyping and random amplified polymorphic DNA (RAPD) genotyping.

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

X. Cao, Wu, Z., Zhou, R., Jiang, F. L., and Yang, Z. E., A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes, vol. 14, pp. 1650-1661, 2015.

Cultivar identification diagrams (CIDs) provide a rapid and efficient approach for identifying cultivars based on random amplified polymorphic DNA (RAPD) markers. In this paper, 64 tomato cultivars were identified using a CID. Using RAPD profiles, clustering analysis was performed to analyze genetic diversity. The results showed that 8 RAPD primers could completely separate the 64 cultivars according to the obtained polymorphic bands; a CID of the 64 tomato cultivars was then constructed.

Genetic diversity and phylogenetic relationship among Tunisian cactus species (Opuntia) as revealed by random amplified microsatellite polymorphism markers

B. M. Zarroug, Baraket, G., Zourgui, L., Souid, S., and A. Hannachi, S., Genetic diversity and phylogenetic relationship among Tunisian cactus species (Opuntia) as revealed by random amplified microsatellite polymorphism markers, vol. 14, pp. 1423-1433, 2015.

Opuntia ficus indica is one of the most economically important species in the Cactaceae family. Increased interest in this crop stems from its potential contribution to agricultural diversification, application in the exploitation of marginal lands, and utility as additional income sources for farmers. In Tunisia, O. ficus indica has been affected by drastic genetic erosion resulting from biotic and abiotic stresses. Thus, it is imperative to identify and preserve this germplasm.

Random amplified polymorphic DNA analysis of salt-tolerant tobacco mutants generated by gamma radiation

Ö. Çelik and Atak, Ç., Random amplified polymorphic DNA analysis of salt-tolerant tobacco mutants generated by gamma radiation, vol. 14, pp. 1324-1337, 2015.

Salinity is one of the major problems limiting the yield of agricultural products. Radiation mutagenesis is used to improve salt-tolerant mutant plants. In this study, we aimed to improve salt-tolerant mutants of two oriental tobacco varieties. One thousand seeds of each variety (M0) were irradiated with 100, 200, 300, and 400 Gy gamma rays by Cs-137 gamma. In the M1 generation, 2999 single plants were harvested. The next season, these seeds were bulked and planted to obtain M2 progeny. The seeds of 1900 M2 plants were picked separately.

Analysis of genetic diversity of salt-tolerant alfalfa germplasms

J. Jiang, Yang, B. L., Xia, T., Yu, S. M., Wu, Y. N., Jin, H., and Li, J. R., Analysis of genetic diversity of salt-tolerant alfalfa germplasms, vol. 14, pp. 4438-4447, 2015.

Random amplified polymorphic DNA technology was used to analyze the genetic diversity of 25 salt-tolerant alfalfa varieties using 30 different primers. Results showed that the percentage of polymorphic loci between single-plant DNA was 81.52%, and that between mixed DNA of various varieties was 61.65%. Compared to the mixed DNA samples, single-plant DNA samples can better reveal the level of genetic variation among and between alfalfa varieties.

Application of molecular markers to detect DNA damage caused by environmental pollutants in lichen species

D. Cansaran-Duman, Altunkaynak, E., Aslan, A., Büyük, İ., and Aras, S., Application of molecular markers to detect DNA damage caused by environmental pollutants in lichen species, vol. 14, pp. 4637-4650, 2015.

Pseudevernia furfuracea L. (Zopf), Peltigera praetextata (Flörke ex Sommerf.) Zopf, Lobaria pulmonaria (L.) Hoffm., and Usnea longissima Ach. lichen species were used as bioindicators to assess the genotoxicity of air pollutants. In the present study, we examined significant environmetal pollutants and investigate how changes may lead to damage in DNA structure using RAPD markers.

Extraction of total DNA and optimization of the RAPD reaction system in Dioscorea opposita Thunb.

G. Q. Wen, Li, J., Liu, X. H., Zhang, Y. S., and Wen, S. S., Extraction of total DNA and optimization of the RAPD reaction system in Dioscorea opposita Thunb., vol. 13, pp. 1339-1347, 2014.

Dioscorea opposita Thunb. has been used as health food and herbal medicinal ingredients in traditional Chinese medicine. In this study, the total DNA of D. opposita Thunb. was extracted using an improved cetyltrimethylammonium bromide (CTAB) method, and the extracted DNA was further used for random amplified polymorphic DNA (RAPD) reaction system by design of the L16 (44) orthogonal diagram.

Molecular characterization of aflatoxigenic and non-aflatoxigenic Aspergillus flavus isolates collected from corn grains

M. A. Mahmoud, Ali, H. M., El-Aziz, A. R. M., Al-Othman, M. R., and Al-Wadai, A. S., Molecular characterization of aflatoxigenic and non-aflatoxigenic Aspergillus flavus isolates collected from corn grains, vol. 13, pp. 9352-9370, 2014.

Twelve species from six fungal genera were found to be associated with corn (Zea mays L.) grain samples collected from three main regions of Saudi Arabia. The average frequencies of the most common genera were Aspergillus (11.4%), Fusarium (9.5%), Penicillium (5.1%), and Alternaria (5.8%). Fifteen isolates of Aspergillus flavus were screened by HPLC for their ability to produce aflatoxins (AF). The percentage of aflatoxigenic A. flavus isolates was 53%. Eight isolates produced AF, at concentrations ranging 0.7-2.9 ppb.

Correlation of genetic variation among wild Trigonella foenum-graecum L. accessions with their antioxidant potential status

E. A. Haliem and Al-Huqail, A. A., Correlation of genetic variation among wild Trigonella foenum-graecum L. accessions with their antioxidant potential status, vol. 13, pp. 10464-10481, 2014.

In this study, we analyzed the correlation between genetic variation based on random amplified polymorphic DNA (RAPD), acid phosphatase, and glutamate-oxaloacetate transaminase isozymes, and amino acid composition with the antioxidant potential status of 7 wild Trigonella foenum-graecum L. accessions collected from diverse ecogeographical regions. RAPD revealed that 90 DNA products had highly polymorphism value (94.12%) based on band numbers, with sizes ranging from 50-2100 base pairs, and band intensity.

Impact of climatic factors on genetic diversity of Stipa breviflora populations in Inner Mongolia

Q. Zhang, Niu, J. M., Wu, S. B., Buyantuyev, A., and Dong, J. J., Impact of climatic factors on genetic diversity of Stipa breviflora populations in Inner Mongolia, vol. 11, pp. 2081-2093, 2012.

Genetic diversity of Stipa breviflora populations in the Inner Mongolian grasslands of China and its possible correlation with climatic factors was examined using geographic information systems and random amplified polymorphism DNA analysis. A total of 308 bands were produced with 28 arbitrary decamer oligonucleotide. Three major findings were demonstrated. First, the genetic diversity of S. breviflora was high but lower than that of Stipa grandis and Stipa krylovii.

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