Development of RAPD-SCAR markers for different Ganoderma species authentication by improved RAPD amplification and molecular cloning
, “Development of RAPD-SCAR markers for different Ganoderma species authentication by improved RAPD amplification and molecular cloning”, vol. 14, pp. 5667-5676, 2015.
The sequence-characterized amplified region (SCAR) is a valuable molecular technique for the genetic identification of any species. This method is mainly derived from the molecular cloning of the amplified DNA fragments achieved from the random amplified polymorphic DNA (RAPD). In this study, we collected DNA from 10 species of Ganoderma mushroom and amplified the DNA using an improved RAPD technique. The amplified fragments were then cloned into a T-vector, and positive clones were screened, indentified, and sequenced for the development of SCAR markers.
