Quantitative real-time polymerase chain reaction

A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression

H. Li, Jiang, Q., Ju, Z. H., Huang, J. M., Wang, X. G., Yang, C. H., Sun, Y., Yang, G. W., Zhong, J. F., Wang, C. F., Li, H., Jiang, Q., Ju, Z. H., Huang, J. M., Wang, X. G., Yang, C. H., Sun, Y., Yang, G. W., Zhong, J. F., Wang, C. F., Li, H., Jiang, Q., Ju, Z. H., Huang, J. M., Wang, X. G., Yang, C. H., Sun, Y., Yang, G. W., Zhong, J. F., and Wang, C. F., “A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression”, vol. 15, p. -, 2016.

The polypeptide N-acetylgalactosaminyltransferase-like protein 5 (GALNTL5) is a newly identified protein that is specifically expressed in testis tissue and participates in spermatogenesis. In this study, we characterized a novel bovine GALNTL5 splice variant, designated as GALNTL5-AS, by using real-time polymerase chain reaction (RT-PCR) and clone sequencing methods. The novel GALNTL5 isoform was derived from the complete transcript, GALNTL5-complete, via alternative splicing (AS). The pattern of the splice variant was exon skipping.

Identification of genes involved in spontaneous leaf color variation in Pseudosasa japonica

H. Y. Yang, Xia, X. W., Fang, W., Fu, Y., An, M. M., and Zhou, M. B., “Identification of genes involved in spontaneous leaf color variation in Pseudosasa japonica”, vol. 14, pp. 11827-11840, 2015.

Spontaneous leaf color variation in bamboo provides the opportunity to study the mechanisms of leaf color formation and the breeding of ornamental bamboos. Despite the fact that many genes are known to be involved in leaf color variation in model plants, molecular mechanisms governing natural leaf color variation in bamboo have remained obscure. This study aimed to identify the genes responsible for the occurrence of such phenomena in bamboo using the suppression subtractive hybridization (SSH) method between green and albino leaves in Pseudosasa japonica f.

Characterization of a TIR-NBS-LRR gene associated with downy mildew resistance in grape

J. J. Fan, Wang, P., Xu, X., Liu, K., Ruan, Y. Y., Zhu, Y. S., Cui, Z. H., and Zhang, L. J., “Characterization of a TIR-NBS-LRR gene associated with downy mildew resistance in grape”, vol. 14, pp. 7964-7975, 2015.

Grapevine downy mildew, caused by Plasmopara viticola, is a devastating disease that results in considerable economic losses as well as environmental damage through the repeated application of fungicides. The nucleotide-binding site leucine-rich repeat gene family functions in plant immunoactivity against various pathogens and pests. In this study, the 5' and 3' ends of the resistance gene homology fragment RGA5 were obtained by rapid amplification of cDNA ends.

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