Quantitative real-time polymerase chain reaction

A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression

H. Li, Jiang, Q., Ju, Z. H., Huang, J. M., Wang, X. G., Yang, C. H., Sun, Y., Yang, G. W., Zhong, J. F., Wang, C. F., Li, H., Jiang, Q., Ju, Z. H., Huang, J. M., Wang, X. G., Yang, C. H., Sun, Y., Yang, G. W., Zhong, J. F., Wang, C. F., Li, H., Jiang, Q., Ju, Z. H., Huang, J. M., Wang, X. G., Yang, C. H., Sun, Y., Yang, G. W., Zhong, J. F., and Wang, C. F., A novel splice variant of the bovine GALNTL5 gene identified in Chinese Holstein bull testis tissue and its mRNA expression, vol. 15, p. -, 2016.

The polypeptide N-acetylgalactosaminyltransferase-like protein 5 (GALNTL5) is a newly identified protein that is specifically expressed in testis tissue and participates in spermatogenesis. In this study, we characterized a novel bovine GALNTL5 splice variant, designated as GALNTL5-AS, by using real-time polymerase chain reaction (RT-PCR) and clone sequencing methods. The novel GALNTL5 isoform was derived from the complete transcript, GALNTL5-complete, via alternative splicing (AS). The pattern of the splice variant was exon skipping.

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