Quantitative real-time polymerase chain reaction

Quantitative real-time polymerase chain reaction as an efficient molecular tool for detecting minimal residual disease in Moroccan chronic myeloid leukemia patients

A. Moumen, Dehbi, H., Kottwitz, D., M. Amrani, E., Bouchoutrouch, N., H. Hadi, E., Quessar, A., Benchekroun, S., Nadifi, S., and Sefrioui, H., “Quantitative real-time polymerase chain reaction as an efficient molecular tool for detecting minimal residual disease in Moroccan chronic myeloid leukemia patients”, vol. 14, pp. 1044-1055, 2015.

Chronic myeloid leukemia (CML) is characterized by BCR-ABL translocation and an increased number and migration of immature myeloid cells into the peripheral blood. The detection limit of the BCR-ABL transcript, particularly after treatment, is controversial.

Expression analysis of Gli1 and Gli2 in different tissues and muscle-derived cells of Qinchuan cattle

Y. Liu, Wang, H., Cheng, G., Jiang, B., and Zan, L., “Expression analysis of Gli1 and Gli2 in different tissues and muscle-derived cells of Qinchuan cattle”, vol. 13, pp. 8767-8775, 2014.

The Hedgehog (Hh) signaling pathway regulates the differentiation of many kinds of cells and plays a critical role in many embryonic and postnatal developmental processes. Gli1 and Gli2 are two transcription factors of the Hh signaling pathway. In this study, we used quantitative real-time polymerase chain reaction to detect the relative expression of Gli1 and Gli2 in 13 tissues from three two-year-old purebred Qinchuan cattle, as well as in different cell populations derived from muscle and different stages of myogenic differentiation of myoblasts.

Identification and characterization of a novel splice variant of the PLCζ1 gene in bull testis tissues

Z. H. Ju, Pan, Q., Zhang, Y., Huang, J. M., Qi, C., Wang, X. G., Li, Q. L., Zhong, J. F., Liu, M., and Wang, C. F., “Identification and characterization of a novel splice variant of the PLCζ1 gene in bull testis tissues”, vol. 13, pp. 9899-9909, 2014.

Phospholipase C zeta 1 (PLCζ1), which transcribes a key protein, has an important function in oocyte activation and embryo development because PLCζ1 can trigger a series of intracellular Ca2+ oscillations in mammals. In this study, a novel splice variant in the testis tissues of adult and fetal Chinese Holstein bulls was characterized by reverse transcription-polymerase chain reaction (RT-PCR) and sequencing analysis.

Molecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab Scylla paramamosain

J. H. Hu, Zhang, F. Y., Jiang, K. J., Fang, Y. B., Wang, J., Zhao, M., Qiao, Z. G., and Ma, L. B., “Molecular characterization of thioredoxin-1 and thioredoxin reductase activity in mud crab Scylla paramamosain”, vol. 13, pp. 10241-10255, 2014.

The thioredoxin (Trx) system consists of thioredoxin reductase (TrxR), Trx, and nicotinamide adenine dinucleotide phosphate (NADPH). TrxR is an NADPH-dependent oxidoreductase. Trx is a ubiquitous small protein with a redox-active disulfide bridge that plays important regulatory roles in some vital metabolic reactions. In this study, a cDNA sequence (SpTrx1) showing high identity to the first Trx gene was isolated from a hepatopancreas cDNA library of the mud crab Scylla paramamosain.

Quantitative real-time polymerase chain reaction