Quantitative polymerase chain reaction

Expression and fast preparation of biologically active recombinant human coagulation factor VII in CHO-K1 cells

W. Xiao, Li, C. Q., Xiao, X. P., and Lin, F. Z., Expression and fast preparation of biologically active recombinant human coagulation factor VII in CHO-K1 cells, vol. 12, pp. 6813-6824, 2013.

Human coagulation factor VII (FVII) plays an important role in the blood coagulation process and exists in micro amounts in human plasma; therefore, any attempt at the large-scale production of FVII in significant quantities is challenging. The purpose of this study was to express and obtain biologically active recombinant FVII (rFVII) from Chinese hamster ovary K1 (CHO-K1) cells. The full-length FVII cDNA was isolated from a HepG2 cell line and then subcloned in pcDNA3.1 to construct an expression vector, pcDNA-FVII. CHO-K1 cells were transfected with 1 μg pcDNA-FVII.

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