qRT-PCR

mRNA expression profiles of heat shock proteins of wild and salinity-tolerant swimming crabs, Portunus trituberculatus, subjected to low salinity stress

X. N. Bao, Mu, C. K., Zhang, C., Wang, Y. F., Song, W. W., Li, R. H., and Wang, C. L., mRNA expression profiles of heat shock proteins of wild and salinity-tolerant swimming crabs, Portunus trituberculatus, subjected to low salinity stress, vol. 13, pp. 6837-6847, 2014.

Challenged by the low salinity, 4 parts per thousand (4 ppt), for 72h, the survivals of swimming crabs (Portunus trituberculatus) were collected as the screened group (SG, tolerant to low salinity). Aiming at identifying the mechanism of low salinity tolerance, quantitative real-time PCR was employed to investigate the expression profiles of 4 HSP genes (HSP60, HSP70, HSP90-1, HSP90-2) in the hepatopancreas of wild (WG) and screened (SG) groups of P. trituberculatus exposed to low salinity (4 ppt).

Development-related expression patterns of protein-coding and miRNA genes involved in porcine muscle growth

F. J. Wang, Jin, L., Guo, Y. Q., Liu, R., He, M. N., Li, M. Z., and Li, X. W., Development-related expression patterns of protein-coding and miRNA genes involved in porcine muscle growth, vol. 13, pp. 9921-9930, 2014.

Muscle growth and development is associated with remarkable changes in protein-coding and microRNA (miRNA) gene expression. To determine the expression patterns of genes and miRNAs related to muscle growth and development, we measured the expression levels of 25 protein-coding and 16 miRNA genes in skeletal and cardiac muscles throughout 5 developmental stages by quantitative reverse transcription-polymerase chain reaction.

Effects of various salinities on Na+-K+-ATPase, Hsp70 and Hsp90 expression profiles in juvenile mitten crabs, Eriocheir sinensis

M. Sun, Jiang, K., Zhang, F., Zhang, D., Shen, A., Jiang, M., Shen, X., and Ma, L., Effects of various salinities on Na+-K+-ATPase, Hsp70 and Hsp90 expression profiles in juvenile mitten crabs, Eriocheir sinensis, vol. 11, pp. 978-986, 2012.

Eriocheir sinensis is a euryhaline crab migrating from sea to freshwater habitats during the juvenile stage. We used quantitative real-time polymerase chain reaction (qRT-PCR) to investigate the gene expression profile of Na+-K+-ATPase, Hsp70 (heat shock protein 70) and Hsp90 in megalopa exposed to salinities of 0, 2, 5, 10, and 15 parts per thousand.

Differential expression analysis of porcine MDH1, MDH2 and ME1 genes in adipose tissues

S. L. Zhou, Li, M. Z., Li, Q. H., Guan, J. Q., and Li, X. W., Differential expression analysis of porcine MDH1, MDH2 and ME1 genes in adipose tissues, vol. 11. pp. 1254-1259, 2012.

Malate dehydrogenases 1 and 2 (MDH1 and MDH2), and malic enzyme 1 (ME1) play important roles in the Krebs cycle for energy metabolism. The mRNA abundance changes of MDH1, MDH2 and ME1 genes were measured across six different adipose tissues from the leaner Landrace and fatty Rongchang pig breeds using quantitative real-time PCR. The mRNA of MDH1, MDH2 and ME1 was more abundant in fatty Rongchang pigs than in leaner Landrace pigs.

Expression of the luteinizing hormone receptor (LHR) gene in ovine non-gonadal tissues during estrous cycle

L. H. Wang, Zhang, W., Gao, Q. X., and Wang, F., Expression of the luteinizing hormone receptor (LHR) gene in ovine non-gonadal tissues during estrous cycle, vol. 11, pp. 3766-3780, 2012.

Luteinizing hormone (LH) is an important glycoprotein hormone that regulates gonadal function in mammals and in turn regulates physiological status changes during the estrous cycle. The function of LH is mediated by luteinizing hormone receptor (LHR).

Molecular characterization and expression analysis of the Lrh-1 gene in Chinese Hu sheep

L. H. Wang, Zhang, W., Ji, J. L., Gao, Q. X., Xiao, S. H., and Wang, F., Molecular characterization and expression analysis of the Lrh-1 gene in Chinese Hu sheep, vol. 12, pp. 1490-1500, 2013.

Liver receptor homologue 1 (Lrh-1) is a member of the nuclear receptor belonging to the second subfamily of the nuclear receptor family 5A (NR5A), also named NR5A2, which is important for lipid homeostasis, embryogenesis, and regulation of aromatics. The present study aimed to understand the sequence of ovine Lrh-1 and the expression traits in reproductive organ tissues. Initially, we cloned Lrh-1 from the liver of Hu sheep through degenerate primer of reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends.

Structure and immune expression analysis of hemoglobin genes from the blood clam Tegillarca granosa

Y. B. Bao, Wang, Q., Guo, X. M., and Lin, Z. H., Structure and immune expression analysis of hemoglobin genes from the blood clam Tegillarca granosa, vol. 12, pp. 3110-3123, 2013.

Hemoglobin (Hb) is the major protein component of erythrocytes in animals with red blood, although it can serve additional functions beyond the transport of oxygen. The blood clam (Tegillarca granosa) is one of the few mollusks that has Hb, although the structure and function of molluskan Hbs remain unclear. We characterized two unique and highly compartmentalized blood clam hemoglobin genes, Tg-HbIIA and Tg-HbIIB, at the molecular level.

Physiological quality and gene expression related to heat-resistant proteins at different stages of development of maize seeds

T. Andrade, Von Pinho, E. V. R., Von Pinho, R. G., Oliveira, G. E., Andrade, V., and Fernandes, J. S., Physiological quality and gene expression related to heat-resistant proteins at different stages of development of maize seeds, vol. 12, pp. 3630-3642, 2013.

We quantified and characterized the expression of heat-resistant proteins during seed development of maize lines with distinct levels of tolerance to high drying temperature. A corn field was planted for multiplication of seeds of different lines, two tolerant and two non-tolerant to high drying temperatures. Harvest of the seeds was carried out at various stages of development and they were then subjected to tests of moisture content, germination, first count of germination, accelerated aging, and cold test.

Differential expression of genes in resistant versus susceptible Gyr x Holstein cattle challenged with the tick Rhipicephalus (Boophilus) microplus

C. S. Nascimento, Machado, M. A., Guimarães, S. E. F., Guimarães, M. F. M., Peixoto, J. O., Furlong, J., Prata, M. C. A., Verneque, R. S., Teodoro, R. L., and Lopes, P. S., Differential expression of genes in resistant versus susceptible Gyr x Holstein cattle challenged with the tick Rhipicephalus (Boophilus) microplus, vol. 9. pp. 1974-1979, 2010.

The bovine tick Rhipicephalus (Boophilus) microplus causes major losses in cattle production systems in tropical regions. Bos indicus breeds are more resistant to ticks than B. taurus breeds. Resistance genes could be an alternative to control this parasite. We examined the pattern of gene expression of three calcium-binding-protein genes: translationally controlled tumor protein 1 (TPT1), allergen Bos d3 (S100A7), calcium channel protein transient receptor potential vanilloid 6 (TRPV6), and the cysteine proteinase inhibitor gene (CST6).

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