Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that affects diarthrodial joints. RA affects ∼1% of the world’s population and is characterized by synovial hyperplasia, infiltration of large numbers of inflammatory cells into the joints, production of autoantibodies, systemic inflammation, and cardiovascular complications (McInnes and Schett, 2011; Arend and Firestein, 2012).
Macrophages are essential components of the innate and adaptive immune responses, playing a decisive role in atherosclerosis, asthma, obesity, and cancer. The differential gene expression resulting from adhesion of macrophages to the extra-cellular matrix (ECM) has been studied in the J774A1 murine macrophage cell line using quantitative polymerase chain reaction (qPCR). The goal of this study was to identify housekeeping genes (HKGs) that remain stable and unaltered under normal culture conditions and in the presence of laminin after a time lapse of 6 and 24 h.
