Pyrosequencing

Rhizosphere bacteriome of the medicinal plant Sapindus saponaria L. revealed by pyrosequencing

A. Garcia, Polonio, J. C., Polli, A. D., Santos, C. M., Rhoden, S. A., Quecine, M. C., Azevedo, J. L., and Pamphile, J. A., “Rhizosphere bacteriome of the medicinal plant Sapindus saponaria L. revealed by pyrosequencing”, vol. 15, no. 4, p. -, 2016.

INTRODUCTION

Sequence analysis of the S1PR1 gene in river buffalo

N. B. Stafuzza, Naressi, B. C. M., Borges, M. M., Amaral-Trusty, M. E. J., Stafuzza, N. B., Naressi, B. C. M., Borges, M. M., Amaral-Trusty, M. E. J., Stafuzza, N. B., Naressi, B. C. M., Borges, M. M., and Amaral-Trusty, M. E. J., “Sequence analysis of the S1PR1 gene in river buffalo”, vol. 15, p. -, 2016.

Recent developments in methodologies for genomic analyses have enabled a significant advance in understanding of the river buffalo genome. The S1PR1 gene has been mapped to buffalo chromosome 6 and bovine chromosome 3; this gene is of interest as it is a candidate for marbling in meat, an important economic trait. Here, we performed next generation sequencing in a buffalo BAC DNA clone and obtained a 54.5-kb sequence encompassing the entire buffalo S1PR1 gene as well as the 27 kb upstream region and the 22 kb downstream region.

Molecular characterization of interferon regulatory factor 1 in Bubalus bubalis

N. B. Stafuzza, Borges, M. M., and Amaral-Trusty, M. E. J., “Molecular characterization of interferon regulatory factor 1 in Bubalus bubalis”, vol. 14, pp. 10919-10928, 2015.

Interferon regulatory factor 1 (IRF1) is functionally diverse in the regulation of immune response and is considered to be an important candidate gene for studying disease susceptibility in mammals. In this paper, we characterized the whole sequence of the IRF1 gene in river buffalo (Bubalus bubalis) and compared genomic and the amino acid sequences between different species. The buffalo IRF1 gene was 7099 bp long and organized into 10 exons and nine introns.

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