Pyrosequencing

Development of novel polymorphic microsatellite markers for the blood clam Tegillarca granosa by pyrosequencing

Y. H. Dong, Yao, H. H., Shi, S. F., Bao, Y. B., and Lin, Z. H., Development of novel polymorphic microsatellite markers for the blood clam Tegillarca granosa by pyrosequencing, vol. 14, pp. 8977-8987, 2015.

Large amounts of expressed sequence tags (ESTs) generated using next-generation sequencing technologies provide a cost-effective and valuable genomic resource for the development of microsatellite markers. In this study, we isolated 115 novel polymorphic microsatellite markers for the blood clam Tegillarca granosa from ESTs in 454 sequencing data. All the loci were characterized in 30 individual clams from a natural population in Xiangshan (Zhejiang Province, China). The number of alleles per locus varied from 2 to 10, with an average of 3.78.

Subscribe to Pyrosequencing