Pyrosequencing

Comparative analysis of the river buffalo somatostatin gene

N. B. Stafuzza, Borges, M. M., and Amaral, M. E. J., “Comparative analysis of the river buffalo somatostatin gene”, vol. 13, pp. 10017-10024, 2014.

The somatostatin protein plays a crucial role in the regulation of multiple biological functions, such as growth, fat deposition, and nutrient absorption in vertebrates. Polymorphisms in the somatostatin gene have been associated with growth traits in livestock species, including cattle and goat. In this study, we conducted complete molecular characterization of the somatostatin gene in Bubalus bubalis (Murrah breed) by sequencing a Murrah BAC clone spanning 72,489 base pairs (bp) in length.

Optimization of dengue virus genome assembling using GSFLX 454 pyrosequencing data: evaluation of assembling strategies

S. M. M. Casseb, Cardoso, J. F., Ramos, R., Carneiro, A., Nunes, M., Vasconcelos, P. F. C., and Silva, A., “Optimization of dengue virus genome assembling using GSFLX 454 pyrosequencing data: evaluation of assembling strategies”, vol. 11, pp. 3688-3695, 2012.

Currently assembling genomes without reference is one of the most important challenges for bioinformaticists all over the world in an attempt to characterize new organisms. The current study has used two dengue virus type 4 (DENV-4) strains recently isolated in Brazil, which have its genomes sequenced using the GSFLX 454 sequencer (Roche, Life Science) by the pyrosequencing method. The GSFLX 454 data were used for testing different genome assembling strategies.

Development of microsatellite markers for a hard-shelled mussel, Mytilus coruscus, and cross-species transfer

J. H. Kang, Kim, Y. K., Park, J. Y., Noh, E. S., Jeong, J. E., Lee, Y. S., and Choi, T. J., “Development of microsatellite markers for a hard-shelled mussel, Mytilus coruscus, and cross-species transfer”, vol. 12, pp. 4009-4017, 2013.

The Korean mussel Mytilus coruscus, an endemic marine bivalve mollusk, is economically important. Its population is currently decreasing due to overexploitation and invasion of a more competitive species, Mytilus galloprovincialis. In this study, microsatellite markers for M. coruscus were developed using a cost-effective pyrosequencing technique. Among the 33,859 dinucleotide microsatellite sequences identified, 176 loci that contained more than 8 CA, CT, or AT repeats were selected for primer synthesis.

Development of microsatellite markers for the kelp grouper Epinephelus bruneus by 454 pyrosequencing and transfer to related species

J. - H. Kang, Yang, S. G., Moon, T. S., Park, J. - Y., and Choi, T. - J., “Development of microsatellite markers for the kelp grouper Epinephelus bruneus by 454 pyrosequencing and transfer to related species”, vol. 12, pp. 5485-5493, 2013.

The kelp or longtooth grouper (Epinephelus bruneus), which inhabits Eastern Asia, is the most economically important of 11 grouper species that inhabit the Southern Sea near Jeju Island in Korea. This species is listed as vulnerable by the International Union for the Conservation of Nature and Natural Resources because of a rapid decrease in its resources. We developed microsatellite markers for E. bruneus using the pyrosequencing technique for applications in resource management and aquaculture.

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