Purification

Immunological characteristics of outer membrane protein omp31 of goat Brucella and its monoclonal antibody

W. Y. Zheng, Wang, Y., Zhang, Z. C., and Yan, F., Immunological characteristics of outer membrane protein omp31 of goat Brucella and its monoclonal antibody, vol. 14, pp. 11965-11974, 2015.

We examined the immunological characteristics of outer membrane protein omp31 of goat Brucella and its monoclonal antibody. Genomic DNA from the M5 strain of goat Brucella was amplified by polymerase chain reaction and cloned into the prokaryotic expression vector pGEX-4T-1. The expression and immunological characteristics of the fusion protein GST-omp31 were subjected to preliminary western blot detection with goat Brucella rabbit immune serum. The Brucella immunized BALB/c mouse serum was detected using purified protein.

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